| To investigate the antihypertension effect of YXQN on Spontaneous Hypertension Rat (SHR) and RHD(2K1C), and search for YXQN's protective effect on SHR heart and kidney. By the gene chip, study the gene express of the heart and kidney and the machannism of the protection. And by the anaesthesia normal dog, observe the effection of the YXQN, in order to find the effection on the system of the heart and the vessel. The results are the following: Result:1. The antihypertension effect of YXQN on Spontaneous Hypertension Rat (SHR) (l)Treating with YXQN one time can reduce the blood pressure of SHR. Onehour after YXQN was treated we observed the effect of reducing blood pressure and the peak is after 2 to 4 hours. The blood pressure reduced significantly after 2 hours and the peak is after 3 hours about 9mmHg. The middle and low dose groups also reduce the blood pressure but not yet to the extent of that of high dose group.(2) Different dose groups of YXQN can reduce the blood pressure of SHR. Two weeks after YXQN was treated continuously, the blood pressure is reduced significantly in high dose group compared with model group. YXQN can prevent the development of blood pressure while rats grows.2. The machanism of the antihypertension effect of YXQNThe effect of YXQN on the RAS of SHR: There is no difference on Ang I in the plasma of all the groups. Ang II of all treated groups reeuced, but there is no significiance compared with the model. The ALD content of high dose of YXQN reduced significiacly compare with the model.3. The protective effect of YXQN on the heart and kedney of the SHR(1) Different dose groups of YXQN can reduce LVW and the effect is significant between high dose group and model group. They also can reduce the ratio of LVW to BW and the effect of middle and high dose groups is significant compared with model group. The effect of high dose group is better than Captopril Tablets group.(2) Different groups can reduce contain of left ventricular myocardial collagen and type I to type III collagen ratio. The effect of middle dose group is significant inreducing contain of collagen. The effect of all groups is significant in reducing ratio of type I to type III collagen.(3) The content of Cr and Ua in the plasma of 22-week-old SHR rose siginificiancly compared with the normal group. Differet dose groups can reduce their content, the high dose and the middle dose is better than the other. The content of Cr of the high dose group reduced signifiacely compared with the model group so did the middle dose group. The content of Ua of the high dose group reduced signifiacely compared with the model group so did the low dose group.(4) The effect of YXQN on the RAS of the SHR kidney. All dose groups of YXQN can signifiancely reduce the content of Ang II in the kidney of SHR compared with the model group. The high and the middle dose groups of YXQN can signifiancely reduce the content of ALD in the kidney of SHR compared with the model group.4.The study of the protection effect of YXQN on the damaging heart and kidney of SHR by the gene expression.(1) The genes latent transforming growth factor binding protein 2 (LTBP-2), glycine methyltransferase (Gnmt) and rat beta-galactoside-alpha 2.6-sialyltransferase in YXQN group were expressed in lower level than in model group. The genes CD36 antigen, heart fatty acid binding protein (Fabp3) and propionyl Coenzyme A carboxylase beta polypeptide (Pccb) in YXQN group was expressed in higher level than in model group. They may play an important role in preventing LVH.(2) The first gene chip showed that 389 genes were difference expession. Among them, 273 genes expressed in low level, while 135 genes expressed in high level. On the second gene chip, the number of difference expession gene was 170. 120 genes expressed in low level, and 50 genes expressed in high level.5. The antihypertension effect of YXQN on RHD (2K1C)Treating YXQN by Mouth one time (lOg/kg), the pressure of the conscious RHD decreased without changes in the heart r...
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