The Experimental Research On Sedation And Antitolerance Mechanism To Acute Somatalgia In Rats With Two Different Electroacupunctures

In this research, through animal experiments on Wistar of acute somatalgia on points of Tsusanli and Sanyinjiao adopting Tail-Flick Latency and the electric-discharge of PIN and PEN as indexes, the difference of sedation with pulse-electroacupuncture in contrast to music-electroacupuncture electroaupuncturing by many times was observed from angles of behavior science and neuroelectrophysiology. And the changes of CCK-8-ir and CCKmRNA in brains of rats were further studied synchronously by means of immunohistochemistry and in-situ-hybridization in order to probe into the relations between AOS and sedation, and attempt to bring to light the probable antitolerance mechanisms of difference electroacupunctures.Result : 1. On behavior science, both of these two electroacupunctures were verified to ease pain effectively, but the music-electroacupuncture was better than thepulse-electroacupuncture. And the effect of the latter declined with the the times of electroacupuncture increasing, and even the antitolerance occurred, while which of the former was lasting, stable, and didn't produce antitolerance. 2. On neuroelectrophysiology, it was further verified objectively though observing the electric-discharge of PIN and PEN at CPU and HPC of rats. 3. On immunohistochemistry, the result showed that the positive CCK-8-ir at CPU and HPC of rats raised with the times of pulse-electroacupuncture adding, while which reduced with the music-electroacupuncture, which showed that CCK has the founction of anti-sedation, and it is maybe one of the reasons of the mechanism of antitolerance that CCK in rats' brains didn't increase after music-electroacupuncture by many times continuously. 4. On in-situ-hybridization, the pulse-electroacupuncture enhanced theCCKmRNA in rats' brains from the second times of electroacupuncture, while the music-electroacupuncture decreased, which further verified the important function of CCK in electroacupuncture antitolerance from angle of molecular biology.The conclusion is that the sound wave as the output-wave of electroacupuncture can overcome antitolerance notably, thus improving the electroacupuncture's curative effect. And it was inferred that the possible antitolerance mechanisms of music-electroacupuncture concern the reduce of releasing AOS, namely, CCK-8 participates antitolerance and is maybe the reason reducing curative effect when electroacupuncture.