Effects Of Cake Fertilizer On The Dynamic Changes Of Plastid Pigments And The Quality Formation During Growth And Physiological Mechanism In Tobacco

Plastid pigments are important natural products in plants and there are two types of the pigments: chlorophyll and carotenoids. In tobacco there are two major chlorophylls: chlorophyll a and chlorophyll b. And there are four major carotenoids: lutein, beta-carotene, violaxanthin, and neoxanthin. It is well-known that carotenoid-degraded products such as beta-ionone, damascenone, beta-damascone, 2,2,6-trimethylcyclohex-2,4-dione and megastigmatrienones, are important to aroma of flue-cured tobacco. Sesame cake fertilizer could improve quality of flue-cured tobacco leaves. The pot and field experiments were carried during 2004-2005 years, and the effects of sesame cake fertilizer on plastid pigments, the aroma, chlorophyll fluorescence parameters and its physiological mechanism in flue-cured tobacco leaves during its growth, we also analysis the effects of cake fertilizers on the relationship between plastid pigments and the system of its physiological mechanism.The details results showed as that:1. A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for the determination on cartenoids in flue-cured tobacco leaves. Carotenoids were extracted from flue-cued tobacco leaves by acetone-water(90:10,v/v) containing 0.1% BHT. Plant protein was eliminated by adding 0.1g Pb(Ac)₂ and centrifuging (10 000r/min, 5min) at 4°C. Lutein, β -carotene, neoxanthin, violaxanthin and other plant pigments were separated on a reversed-phase C₁₈ column(3.9mm i.d. × 150mm, 5μm), with a mobile phase of (A) methanol-isopropyl alcoho(1:1,v/v) and (B) water using a gradient elution at a flow rate of 0.5mL/min. The optimum elution gradient was: 0-10min(70%A+30%B), 10-17min(100%A), 17-30min(90%A + 10%B). The recovery of carotenoids in flue-cured tobacco leaves were 91.77%-102.8%, and relative standard deviations were...