| Amur sturgeon (Acipenser schrencki Brandt) is a kind of essential economic species in Heilongjiang River and now become an important culture fish. It is very useful to study the immunological factors and other related factors of the fish for curing and preventing diseases and adopting immunization techniques in the fish culture.Blood cells are important in immune system. Reticulocyte and erytnrocyte which are splitting were found There are four kind of leukocytes in peripheral blood of Amur sturgeon: lymphocyte, granulocyte, tnrombocyte and monocyte. Blood cells of the fish were observed under electron microscope. There are a little organells in the cytoplasm of the erythrocyte. The lymphocyte is typical and there are lots of mitochondria in cytoplasm of the monocyte which is smaller than granulocyte. Thrombocyte is in fusiform or nearly round form with little cytoplasm. The granules of granulocyte are classified into two kinds based on electron-lucent and electron-dense.Serum protein and Ig of 2+ years old Amur sturgeon, 3+ years old Chinese sturgeon Acipenser sinesis Gray and 3 years old Huso sturgeon Huso dauricus Georgi are analyzed. The results show that serum proteins of the three fishes are different with each other in composition and level of serum proteins. Coefficient of similarity about serum protein show that Chinese sturgeon is more similar with Amur sturgeon compared with Huso sturgeon. Saturation ammonium sulfate sedimentation and Sephadex G-200 gel chromatography were used to purify Ig from serum of Amur sturgeon, Chinese sturgeon and Huso sturgeon respectively. IgM molecular weight of Amur sturgeon, Chinese sturgeon and Huso sturgeon are 867KD, 896KD and 924KD respectively assayed by unreduced PAGE. Reduced SDS-PAGE shows that heavy chain molecule weight of IgM in the three Sturgeons serum are all 88KD and light chain molecule weight of Amur sturgeon and Chinese sturgeon are 29KD, while there are two kinds of light chain in Huso sturgeon with molecule weight at 29KD and 26KD. Western-blotting analysis showed antibody activity of IgM and the heavy chain of IgM in serum of die three Sturgeons. Ouchterlony results show that IgM of each of the three Sturgeons can precipitated with rabbit serum anti IgM of each other, but cannot response with rabbit serum anti carp IgM. IgM of the three Sturgeons can be hydrolyzed by papain in corresponding conditions and two fragments are obtained. Molecule weight of first peak is 66KD and that of the second is 44 KD determined by SDS-PAGE. Content of IgM in sera of Ihe three Sturgeons were assayed by sandwich ELISA with sensitivity at 16ng.ml-1.C3 content in serum increased significantly while the fish infected by Aeromonas hydrophila (Ah) and C4 showed insignificant in the same condition It seemed that C3 is important in Ahinfection. Lysozyme content in serum and other tissues of 1+ years old Amur sturgeon tended to increase with the temperature, but increasing range varied in different tissues. Lysozyme content in serum, mucus, liver, stomach and hindgut showed significant difference between the lowest and the highest temperature in the test while that in the other tissues showed no significant difference. We analyzed distribution of lysozyme in serum and several tissues of Amur sturgeon and the results show that lysozyme content in foregut is the highest in the tissues assayed in every temperature in the test while lysozyme in pylonc caecum, hindgut and spleen show hight level in the test and that in serum and mucus are lower in level compared with the other tissues. Lysozyme content in serum increased insignificantly after Amur sturgeon infected by Ah compared with that in the normal fish. Lysozyme in liver showed significant increasing between normal fish and fish infected except at 14-15℃. Difference of lysozyme in stomach, mucus and gill between nonnal fish and fish infected varied in different temperature. Lysozyme in foregut, hindgut and pylonc caecum decreased after the fish infected by Ah. The changes of different tissues were not the same after... |
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