| Objective Pollinosis is an allergic disease caused by plant pollen,which has obvious seasonal and regional characteristics.As the major airborne allergen in northern China,seven component allergens have been identified.The purpose of this study was to analyze the sensitization patterns of three major components of Artemisia in Inner Mongolia,China,and to evaluate the clinical diagnostic value of artemisia pollen components.In addition,this study analyzed the association between sensitization of Artemisia annua components and clinical symptoms,as well as the correlation with common weed/tree pollen allergy.Methods 1 Indirect ELISA was established by coating three main allergic components(Art v1、Art v 3、Art v 4).Artemisia positive and negative serum was added as the primary antibody.Mouse anti-human Ig E Fc monoclonal antibody(horseradish peroxidase labelled)was added as the secondary antibody.The working conditions were screened and optimized to establish the component-resolved diagnosis methods,and to determine the positive rate of s Ig E against the three components.At the same time,the influence of each component antigen on artemisia pollen allergy was evaluated by binary logistic regression.The ROC curve was established to evaluate the diagnostic efficiency of the single artemisia pollen component.In order to further optimize the efficiency of the test,the ROC curve of multiple variables was used to measure the diagnostic performance of multi-component antigens.2 The sIgE levels of crude extract and four components of A.annua were determined by protein chip technology in patients with severe allergic rhinitis,and the relationship between sensitization of each component and asthma was analyzed.The correlation between s Ig E to A.annua components and common weed/tree pollen allergies was explored by Pearson correlation analysis.Results 1 After exploration,the three components of Artemisia were selected to be coated at a concentration of 1μg/ml,the tested serum was diluted at a dilution of 1:2,and mouse anti-human Ig E Fc antibody was selected at a dilution of 1:3000.Then,the serum of 82 artemisia-allergic patients and 32 healthy people were detected.The results showed that the Ig E binding rates of Art v 1,Art v 3 and Art v 4 were 70.7%(58/82)、53.7%(44/82)、42.7%(35/82),respectively.Among the three components,Art v 1 had the largest AUC(0.883)that showed the best diagnostic performance.The optimal cut-off value determined by ROC analysis was 0.086,with a sensitivity of 72.0% and a specificity of 96.9%.The diagnostic efficiency was highest for the combination of Art v 1+Art v 3+Art v 4,which performed an AUC of 0.939.2 All the individuals showed s Ig E reactivity to the whole extracts of A.annua.Sensitization prevalence to Art an 1,Art an 2,Art an 3 and Art an 4 were 83%(48/58),69%(40/58),57%(33/58),and 41%(24/58)respectively.Levels of s Ig E to Art an 3 were significantly higher in asthmatic individuals than those with rhinitis alone(Z=-3.256,p<0.05).Allergy to artemisia was positively correlated with the plane tree,elm,ragweed,dandelion and grass pollen sensitization(r=0.388,0.519,0.464,0.571,and 0.275,respectively,p<0.05).Regarding artemisia allergens,s Ig E levels to Art an 4 were strongly correlated with the plane tree,elm,ragweed and grass pollen allergy(r=0.908,0.76,0.864,0.838,p < 0.05).Conclusion CRD is available for estimating the molecular sensitization profile in artemisia-allergic patients.Combinations of artemisia components with the application of the ROC curve could improve diagnostic performance.There are inter-individual variations in the sensitization to various artemisia allergens.Sensitization to artemisia components has extensive cross-reactivity with weed/tree pollen allergy. |
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