Phenotypic Study On The Effect Of Periodontal Pathogens On Total Bonemineral Density & Case Report

Aim :periodontitis is one of the most popular diseases through china and even the whole world,associated with many systemic disorders.Periodontal pathogenic bacteria could traffic through blood circulation to other tissues and organs,leading to multiple abnormalities.Epidemiologic investigation reveals a high relevance between periodontitis and osteoporosis.Using animal experiments,this article aimed to confirm that periodontal pathogens could contribute to osteoporosis via blood circulation.Methods: Eighteen female SD rats(16 weeks)were divided into two groups named control group(9 rats)and periodontitis group(9 rats),in which the rats were treated with periodontal ligation.The ligation silk,total blood and blood serum were collected periodically.ELISA was used to test inflammatory cytokines within blood.PCR was carried out to detect periodontal pathogenic bacteria on ligation silk as well as blood.The status of global bones was measured by Micro CT,TRAP staining and HE staining.Results: The rat periodontitis model was successful as Pg,Aa and Fn successfully accessed into blood.Rats in experimental group exhibited a higher level of IL-6 and TNF-a compared with control group(p<0.05).Results from Micro CT and HE staining showed a decrease of bone density in rats with periodontitis(p<0.05).TRAP staining results indicated a significant increase of osteoclasts through global bone tissues(p<0.05).Conclusion: Periodontal pathogenic bacteria P.g A.a and F.n cause an expansion of global IL-6 and TNF-a,a reduction of global CTX-1 through blood circulation,the consequence of which may be related to the reduction of bone density.