Research On Syndrome Of Dampness In Middle Jiao Model Affecting Aquaporins In Liver By Pingwei Powder

Objective:The anatomy of the liver is an important organ for digestion and metabolism.It has a high correlation with the functions of the"spleen controlling digestion"in traditional Chinese medicine."Transportation"includes the function of transporting water,so the function of liver aquaporins and traditional Chinese medicine"fortify the spleen and disinhibit dampness"and"dredge water"are closely related.In this experiment,we systematically studied the distribution,quantification,activity,and regulation of AQP-1 and AQP-4 in rat livers with dampness resistance,and explored the effects of coke in dampness on AQP-1 and AQP-4.Whether Ping wei powder affects the expression of aquaporin or not,regulates the state of fluid metabolism,and reveals that some of the molecular mechanisms that lead to the imbalance of cellular fluid metabolism in the damp-resistance caused by syndrome of accumulation of dampness in middle-jiao and peace gastric dispersal play a role in the regulation of cell transmembrane water transport.The reason why AQP-1 and AQP-4 were selected as the study was:the previous experimental study found that AQP-1 and AQP-4 were expressed in the liver,lung,kidney,and gastrointestinal,which are co-expressed aquaporins,and this Several organs are closely related to the fluid metabolism,because other organs have been studied in the early stage^[1],and the AQP-1 and AQP-4 studies in the liver have been selected in order to form multiple organs to evaluate together.In order to study the expression patterns of aquaporins and the effects of wet-induced coke on aquaporins,this study specifically selected these two proteins for exploration.Method:Adopting the previous mature 20-day modeling method of our research group,we establish the rat model with Syndrome of dampness in middle Jiao.By measuring the rate of gastric remnant and intestinal propulsive rate in rats,we chose the phloroglucin to determine the content of D-xylose,chemical colorimetry to measure the activity of Na⁺-K⁺-ATP in liver.Enzyme linked immunosorbent assay（ELISA）to determine the contents of AQP1 and AQP4 in liver tissue,and immunohistochemistry SP,mean optical density,PCR and aquaporin phosphorylation to detect the expression and distribution of AQP1 and AQP4 in liver tissues.Results:1.The ment of the model with Syndrome of dampness in middle Jiao:1.Through general observation,the weight of rats was significantly reduced,the amount of feeding and drinking intake decreased significantly,and the mental state was poor in the model group.2.Objective index showed that rats in model group arised gastric emptying disorder,serum D-xylose content and Na⁺-K⁺-ATP enzyme activity decreased.3.After having been treated with Pingwei Powder,the feeding and drinking intake,weight,gastric remnant rate,intestinal propulsive rate,serum D-xylose absorption and Na⁺-K⁺-ATP enzyme activity were improved in the model group,speculating that the rat model with Syndrome of dampness in middle Jiao was successed established。2.Liver protein content:After modeling,compared with the blank group,the content of aquaporins in the model group decreased:liver AQP1,AQP4 and Na⁺-K⁺-ATP enzymes.After the intervention of Pingwei Powder,compared with the natural recovery,the content of aquaporins in the Pingwei Powder group was increased:liver AQP1,AQP4and Na⁺-K⁺-ATP enzymes.After natural recovery,compared with the model group,the content of water channel protein in the natural recovery group increased:the liver AQP1,the water channel protein in the rats decreased in the natural recovery group:the liver Na⁺-K⁺-ATP enzyme.3.Expression and distribution of liver protein:After modeling,compared with the blank group,the expression of aquaporins in the model group increased:liver AQP1 and AQP4.After the intervention of Pingwei Powder,compared with the natural recovery,the expression of aquaporins in the Pingwei Powder group was down regulated:liver AQP1 and AQP4.After natural recovery,compared with the model group,the expression of rat water channel protein was increased in the natural recovery group:liver AQP1,and the expression of aquaporin in the natural recovery group was decreased:liver AQP4.Conclusion:Syndrome of dampness in middle Jiao can not only affect the energy metabolism,but also affect the protein content and expression level of liver AQP1 and AQP4,which may be one of the mechanisms leading to the imbalance of the water metabolism in Syndrome of dampness in middle Jiao;Pingwei Powder can coordinate the activity of Na⁺-K⁺-ATP enzyme and the content and distribution of AQP1 and AQP4 in liver,and adjust the energy metabolism and water metabolism regulation,which may be one of the mechanisms of Pingwei Powder of drying wet of the spleen.