| Objective: To investigate the accumulation of rat with model of syndrome of dampness in middle Jiao of anaerobic glycolysis and gluconeogenesis and glucose metabolism.To reveal the partial molecular mechanism of metabolic and energy metabolic imbalance of the dampness syndrome,and the effect of the spleen and stomach on the regulation of glucose metabolism and glycolysis and glycolysis and the intervention of Pingwei powder and patchouli positive gas.Method: the experimental model of the early mature wet resistance in middle Jiao,establish the animal model of dampness obstructing spleen stomach syndrome.The content of D-xylose in serum was detected by three phenol method.The activity of liver Na+-K+-ATP enzyme was detected by chemical colorimetry.The content of glucose in serum,liver and muscle was detected by peroxidase method.The content of protein,pyruvic acid,lactic acid,liver glycogen in liver tissue and the content of lactic acid,insulin,glucagon in blood and the content of lactic acid and muscle glycogen in the blood were detected by chemical method,and the content of lactic acid and muscle glycogen in muscle,E,and muscle glycogen content,and the content of lactic acid and muscle glycogen in muscle;The content of hexokinase,pyruvate kinase,fructose kinase-1,2,6-diphosphate fructose,acetyl coenzyme A,pyruvate carboxylase,pyruvate carboxylase,phosphoenolpyruvate carboxylase,glucose-6-phosphatase,and lactate dehydrogenase and pyruvic acid in muscle were detected by LISA,and the content of ATP was detected by HPLC.Results: 1.The establishment of 1 models of wet resistance in middle JiaoThrough general observation,compared with the blank group,the rats in the model group were significantly reduced in weight,diet and mental state.(2)objective index test showed that gastric emptying delayed,serum D-xylose content decreased and Na+-K+-ATP enzyme activity decreased in model group.(3)after treatment,the body weight,mental state,gastric remnant rate,intestinal propulsive rate,D-xylose absorption and Na+-K+-ATP enzyme activity were improved in the model group wet model of middle energizer resistance rats.2.The changes of glucose,glycogen,ATP and related factors in rats with syndrome of dampness in middle JiaoThe glucose content of glucose,liver glycogen,liver tissue ATP and muscle tissue in the serum and liver tissues of the model group was significantly higher than that in the blank group,but the serum insulin,glucagon content and the ratio of insulin to glucagon were decreased,and the myosin was not significantly changed.Compared with the model group,the glucose content,liver glycogen,liver tissue ATP and muscle tissue in the serum and liver tissues of the natural recovery group decreased significantly.The serum insulin,glucagon content and the ratio of insulin to glucagon continued to decrease,and there was no significant change in the muscle glycogen,and the content of ATP natural recovery group was higher than that of the blank group.The content of glucose,ATP,liver glycogen and muscle tissue in liver tissue decreased obviously.The levels of glucose,liver sugar,liver sugar,After Pingwei powder,low dose of serum glucose and low dose group and low dose group were lower than those in the blank group,and the ratio of insulin to glucagon decreased.The level of insulin and glucagon in the middle and low dose group was lower than that in the blank group,and the content of insulin and glucagon in the serum of the rats decreased continuously,the change trend of the two was the same and that the lower dose of the stomach was the most obvious.The ATP content of glucose,glucagon,liver glycogen and liver tissue in the serum of rats decreased,and the glucose content in the muscle tissue was not significantly changed,but the glucose content in the liver tissue decreased and the dose was negatively related to the dosage of the drug.The muscle glycogen,insulin content and the ratio of insulin to glucagon were all increased,among them liver glycogen and serum glucosamine The average level of water in each group was lower than that in the blank group.The level of the medium dose group and the low dose group of the insulin and the insulin and glucagon ratio was higher than that of the blank group.3.The changes of pyruvic acid content in liver of rats with syndrome of dampness in middle JiaoCompared with the blank group,the content of pyruvic acid in the liver tissue of the model group was significantly lower than that in the blank group.Compared with the model group,the content of pyruvic acid in the liver tissue of the natural recovery group was higher than that in the blank group.The level of pyruvic acid in liver tissue was higher than that in the blank group,and the level of pyruvic acid in liver tissue was higher than that in the blank group.4.The changes in related factors of anaerobic glycolysis in rats with syndrome of dampness in middle Jiao4.1The changes in related factors of anaerobic glycolysis in rats with syndrome of dampness in middle JiaoCompared with the blank group,the content of hexokinase,pyruvate kinase,fructose kinase-1,2,6-diphosphate fructose decreased or decreased in the model group.Compared with the model group,the content of hexokinase,pyruvate irritable enzyme,fructose kinase-1 and 2,6-diphosphate was increased in the natural recovery group,including pyruvate kinase and phosphorus.Cranberry sugar kinase-1,2,6-diphosphate fructose natural recovery group was higher than that in blank group.The level of hexokinase in liver tissue was lower and negative correlation with the dosage,while the content of pyruvate kinase,fructose kinase-1 and 2,6-diphosphate in natural recovery group was higher than that in the blank group,and the level of hexokinase and 2,6-diphosphate fructose in liver tissue was higher than that in the blank group.The content of Phospho fructose kinase-1 decreased.4.2 The changes of lactic acid cycle related factors in rats with syndrome of dampness in middle JiaoCompared with the blank group,the lactate content in the liver and muscle of the model rats increased,the lactate content in the serum decreased,the content of pyruvic acid and lactate dehydrogenase in the muscle tissue decreased and the lactate dehydrogenase content in the liver tissues increased.Compared with the model group,the lactate dehydrogenase in the muscle tissue was contained in the lactate dehydrogenase in the serum and the liver tissues.The content of lactic acid dehydrogenase in liver tissue and muscle tissue was higher than that in blank group,and the lactate content in muscle tissue was lower than that of blank group.Compared with the model group,the content of lactic acid in the liver and serum increased,the content of lactic acid and pyruvic acid in the muscle decreased,the content of lactate dehydrogenase in the liver and muscle increased,and the content of lactate in the liver and muscle decreased,the content of lactic acid in the liver and muscles decreased,the content of lactic acid in the muscle,muscle and lactic acid decreased,and the lactic acid in the muscle,lactic acid,lactic acid,lactic acid,and lactic acid were lower in the model group.The content of lactate dehydrogenase in liver tissue increased with the increase of dehydrogenase content.5.The changes of related factors in the gluconeogenesis pathway of rats with syndrome of dampness in middle JiaoCompared with the blank group,the content of pyruvate carboxylase in the liver tissue of the model group increased,the content of phosphoenolpyruvate carboxylic kinase decreased,and the content of glucose-6-phosphatase remained stable.Compared with the model group,the content of pyruvate carboxylase and phosphoenolpyruvate carboxykinase in the natural recovery group increased,and the content of glucose-6-phosphatase still had no effect obvious changes.After the intervention of Pingwei powder and Huoxiangzhengqi Powder,the pyruvate carboxylase,phosphoenolpyruvate carboxykinase and glucose-6-phosphatase contents in liver tissue increased.Conclusion: The model of dampness resistance in the middle of coke can affect the partial related factors of glucose metabolism and glycolysis,which may be one of the mechanisms of the imbalance of substance metabolism and energy metabolism in the body of wet hindrance and middle coke.With the regulation of material metabolism and energy metabolism,it may be one of the targets of regulating glucose anaerobic metabolism in the spleen and stomach,and one of the mechanisms of the action of stomach and stomach.Pingwei powder and Huoxiangzhengqi Powder consistent effect trend of accumulation of dampness in middle Jiao,but different. |
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