Establishment Of The Micropropagation System Of Cotoneaster Salicifolius

Cotoneaster salicifolius is a plant of the Cotoneaster Rosaceae,it not only has a beautiful shape,but also has a lot of red fruit.Now the demand for garden plants is very large,especially the need of fruit ornamentals.Only by seeding to expand the amount of Cotoneaster salicifolius can not meet the needs of the garden.There are many studies of Cotoneaster salicifolius in the article including axillary bud induction,subculture and domestication and the experimental material is seeding of Cotoneaster salicifolius.The results indicate:1.The most suitable sterilization method is:first use the concentration of 70%alcohol for 30s,and then with the concentration of 4%sodium hypochlorite for 5min.The browning rate is relatively low,the survival rate can reach more than 56%after sterilization.2.In the first generation culture,6-BA had a greater effect on axillary bud induction.The suitable primary medium was MS+6-BA0.8mg/L+NAA0.02mg/L+PPM1.2mg/L.3.Different plant growth regulators and basic culture medium had an effect on the proliferation and culture of tissue culture seedlings.6-BA plays an important role in the differentiation of shoots.MS is a suitable basic medium.The optimum culture medium for the subculture of Leymus chinensis was MS+6-BA 1.0mg/L.4.Tissue culture is relatively small after subculture.So it is not suitable for rooting directly,need to first rooting seedlings cultivation.The most suitable combination of seedling medium is 1/2MS+NAA0.1mg/L.5.Different basic culture medium and growth regulators had an effect on the rooting of Cotoneaster salicifolius seedlings.The best rooting medium was F+NAA0.05mg/L.6.The seedling culture before transplanting can promote the elongation and growth of the stems of the tissue culture seedlings.7.It is necessary to cultivate before transplanting,but pay attention to control the time of the cultivation.The best way to cultivate the seedlings is to close the bottle for 7 days,and then open the bottle for 4 days.8.The type and proportion of transplanted substrate also had a great influence on the survival rate of transplanting.The best transplanting substrate is:vermiculite:peat soil:perlite = 2:1:1.