| Taxus Media is the precious tree with very high ornamental value and economic value, and it is concerned about containing paclitaxel of anti-cancer activity, However, this genus grows slowly and its natural regeneration is difficult. Therefore, Taxus has attracted considerable attention of its conservation and regeneration. While establishing and improving the technique of tissue culture in Taxus is the important way to solve the shortage of resources and other issues.In this paper, tender stems of Taxus Media were used as experimental materials, the best sterilization methods of explants, the best medium of axillary buds induction, the impact factors of browning and the best proliferation medium of adventitious shoots were systematically studied. SAS software is used to analyze data. Through the above tests, we want to study the main effected factors of tissue culture and the best medium in various stages of Taxus Media comprehensively, select the optimal technical parameters, and improve technology system in vitro of Taxus Media plants gradually. Therefore, to realize the industrial production of Taxus and protect the existing resources of species.This research obtains following results:1. the optimum scheme of sterilization for explants of Taxus Media is:the explants of Taxus Media→dipped in the detergent water about 20 min and used a soft brush to clean the surface dirt→washed about 30 min by tap water→putted on the extra-clean desk→dipped in 70 % alcohol 20 s Cotton to clean→washed 2 times by asepsis water→dipped in the0.3 % HgCl26min→washed 8 times by asepsis water→water dried and the bracteal leaves sweeped away→cut the small stems of about 1.5 cm→inoculated. the success rate is 83.3 %.2. The best inducement medium is MS+6-BA 1.0 mg-L-1+NAA 0.4 mg-L-1+AC 0.5 g-L-1+sugar 30 g-L-1+ agar 8.0 g-L-1, pH 5.8-6.0.3. Continuous culturing(without any hormones in MS basic medium) for two weeks in blank experiments can reduce browning rate effectively. but the contamination rate has also increased, so without any pretreatment of Taxus Media is more suitable for the next experimental. The activated carbon concentration on browning of Taxus Media explants were affected significantly, Adding 0.4 g-L-1 of activated carbon is the best way to inhibit the browning of explants. However, the effect decreased gradually as the concentration of activated carbon increased. The best concentration of PVP is 300 mg-L-1 to prevent explants browning. The best concentration of VC is 0.3 g-L-1 to prevent explants browning. To shorten switching cycle cann't be to reduce the occurrence of browning,25 days is the best switching cycle.4. The main factors of bud lumps multiplication and the length of buds are 6-BA,TDZ,NAA,Hydrolyzed milk protein,carbon source and agar.6-BA with 0.15 mg-L-1and NAA with 1.0 mg-L-1can improve the multiplication of bud clumps. The best multiplication medium is MS+6-BA 0.15 mg-L-1+TDZ 0.01 mg-L-1+NAA 1.0 mg-L-1+AC 0.4 g-L-1+LH 200 mg-L-1+sugar 30 g-L-1+agar 8.0 g-L-1, pH 5.8.
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