| The stem of Silvervein Creeper and Virginia Creeper was used as explant to establish the rapidly multiplication technique systm of tissue culture, In addition still utilized the leaf and stalk passage and the petiole of Silvervein Creeper and Virginia Creeper to induction callus and multiplication .With single factor and orthogonal design and analysis of variance, the study aims to find out the optimum explant and best medium composition and the culture process in Silvervein Creeper and Virginia Creeper. The results were as follows:1 The optimum sampling period of Silvervein Creeper and Virginia Creeper is April and July, the germinating rate is high, and grows quickly; The axillary bud on the length location of the body with the below 3-7 of terminal bud is best explant, and pollution frequency is low, the germinateing rate is high, and the seedling grows robustly after survival.2 The optimum explant of Silvervein Creeper to adduction callus is the petiole, the main adduction hormone is NAA ,culture medium : 1/2 MS + NAA 0.1 mg/L + BA 0.5 mg/L + white sugar 30 g/L + agar 7.5g/L, adduced callus by it more early, quantity far more , the greener, differentiatia frequency higher; The optimum explant of Virginia Creeper to adduce callus is the stalk passage, the culture medium constitutes: 1/2 MS + 2,4-D 0.8 mg/L + BA 0.5 mg/L + white sugar 30 g/L + agar 7.5 g/L, the callus is light green, the quality of the callus is loose , differentiatia frequency higher .3 In differentiatia and subculture stage, Silvervein Creeper easy brown, and attachs PVP 300 mg/L alleviate the brownization degree. The fundamental culture medium of more fit growth of Silvervein Creeper is 1/2MS and GS, that attachs BA 0.5 mg/L, and NAA 0.06 mg/L; but Virginia Creeper are living in takes 1/2 MS as the fundamental culture medium to grow better.4 The optimum plant hormone to Virginia Creeper is chooseed, three kinds of growth hormones is NAA, IBA and IAA, and NAA is the most suitable hormone, the lower concentration of it in reproduces stage, NAA 0.02 mg/L~0.08 mg/L is connected with the cytokinin which may accelerate seeding growth; The cytokinin that the adduced multiplication is BA act as goodly, coordinates the NAA 0.02 mg/L~0.08 mg/L the multiplication coefficient attainability up 3.5 when the concentration is 1.5 mg/L, The capability that KT's guidance is reproduce is infirm, but below the concentration KT may lead rooting.5 The Virginia Creeper is very vast as to the accommodation of pH, and is living in the environment of pH 4.6-6.4 wholly to grow, the most fit limit is pH 5.4-6.2 where the seeding grows robustly, and stalk extending amount is greatly; Ammonium nitrogen and the saltpetre nitrogen concentration height also affects stalk extending amount and multiplication coefficient of Virginia Creeper in the fundamental culture medium, and overtops either overlow are all not to be fit for.6 With the plant hormone is connected with organic accretion orthogonal design test, being fit for Virginia Creeper reproduces quickly optimum culture medium: 1/2 MS +NAA 0.04 mg/L + BA 1.0 mg/L + KT 2.0 mg/L + LH 100 mg/L + white sugar 30 g/L + agar 7.5 g/L; 1/2 MS + NAA 0.08 mg/L + BA 1.5 mg/L + KT 1.0 mg/L + LH 100 mg/L + white sugar 30 g/L + agar 7.5 g/L, after culture 30 days, the multiplication coefficient attainability up 3 and grows robustly, it is taller to reproduce the factor attainability.7 In adduction rooting stage, the optimum culture medium adduction rooting: 1/2 MS + IAA 0.5 mg/L + IBA 0.3 mg/L, 1/2 MS + NAA 0.06 mg/L + IBA 0.5 mg/L, at the same time attachs agar 7.5 g/L and sugar 20 g/L the rate of indduction rooting leading to 100%, the seeding high 2.0 cm, brings two leaves blade, the better stuff and the better redduction rooting.8 Acclimatization and transplants, the more suitable acclimatization time is 10 days, and the survival rate after 30 days attains 80% and the transplanting survival rate may more high with prolongs the acclimatization time.Transplanting basic matter adopts vermiculite or ve...
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