Screening Differentially Expressed Genes And Study On The Toxicological Effects In Human Umbilical Vein Endothelial Cells After Exposed To HTO

Objective:To investigate the expression of differentially expressed genes in experimental group compared with the control group after exposed to HTO.Observe changes in cell morphology?size?proliferation and senescence,and estimate the dose absorbed by cells,providing a theoretical basis for the possible damage to vascular endothelial cells caused by internal exposure of HTO.Method:1.HUVEC cells were treated with 3.7?103 Bq/ml HTO for 48 h,RNA was extracted from the experimental and control group.Genes that were differentially expressed between the experimental and control group were analyzed by gene chip technology.The Go and Pathway analyses were used to define and describe the function and possible signal pathways that differential gene may involved in.String 10.5 online analysis tool was used to predict the interaction of the differential genes,and the hub genes were screened out.2.HUVEC cells were continuously exposed for 15 generations with 3.7?103 Bq/ml HTO.The 3rd?6th?9th?12th and 15 th generation of irradiated cells and unirradiated cells were collected,marked as T0?T3?T6?T9?T12?T15.3.After exposure to HTO,the morphology of the cells resuscitated for 24 h,48h and 72 h was observed and recorded under the ordinary optical microscope,and Image J software was used to measure the surface area of the cells.4.After exposure to HTO,the proliferation activity of HUVEC cells resuscitated for 24 h,48h and 72 h was detected using CCK-8 cell proliferation and toxicity assay.5.The ?-galactosidase staining kit was used to detect the cell senescence of HUVEC cells after exposed to HTO.6.The COOLER algorithm was used to estimate the radial distance through the code PARTRAC.Monte Carlo simulation was performed on the electronic traces of liquid water,and the dose absorbed by the cells exposed to HTO was calculated.Result:1.After exposed to HTO,the expression of CTH?CCNE1?BAX?CASP8?RB1 genes was significantly up-regulated,and the expression of RPS27 A and KISS1 genes was significantly down-regulated.2.After exposed to HTO,the morphology of HUVEC cells has been changed with the phenomenon that the number of cytoplasmic particles increased and the boundary was blurred.Cell senescence was observed in T3 and T6 cells.T6?T9?T12?T15 cells proliferation activity was inhibited.With the increase of exposure time,the absorbed dose of cell induced by HTO increased.Conclusion:1.CTH,CCNE1,BAX,CASP8,RB1,RPS27 A,and KISS1 genes may be involved in the process of internal irradiation damage to vascular endothelial cells by HTO.2.HTO has cytotoxicity on HUVEC cells,and with the increase of irradiated generation,the inhibition effect of cell proliferation is more pronounced.