The In Vitro Functional Research Of C-Phycocyanin On Lung Cancer Cells Proliferation

C-phycocyanin(C-PC) is one of the allowable natural food colorants in our country.It is also a kind of important functional food colouring.The objective of this study was to investigate the effect of C-PC on non-small cell lung cancer A549?H1299?H460 and LTEP-a-2cells.phycocyanin could inhibit the proliferation and migration of lung cells,we measured the survival rate and growth curve of lung cancer cells and found that phycocyanin can significantly reduce cell viability,indicating that phycocyanin can inhibit the proliferation of lung cancer cells.In order to further explore how phycocyanin inhibits the proliferation of cells,the cycle distribution of phycocyanin-treated cells was examined by flow cytometry,phycocyanin could regulate the expression of check point-related genes and cause G1 or S phase arrest.To analyze the population-dependent and proliferative abilities of lung cancer cells,we observed the colonies of the lung cancer cells after phycocyanin treatment and found that phycocyanin can reduce lung cancer cell independent viability and environmental adaptability.We also explored the ability of lung cancer cells to migrate in vitro.We found that the migration ability of the cells after phycocyanin treatment was reduced by the scratch repair test,indicating that phycocyanin inhibits the migration of cells in vitro,Then we detected the expression of migration-related genes using western-blot,and found that phycocyanin can significantly reduce protein levels of mmp2 and mmp9.Phycocyanin can cause abnormal morphological changes of lung cancer cells,we initially identified as phycocyanin can cause cell apoptosis.To determine whether phycocyanin caused cell apoptosis,we used flow cytometry to examin the apoptosis of phycocyanin-treated cells and found that the phycocyanin-treated cells showed a significant increase in the early and late apoptotic rates.phycocyanin also induced cell apoptosis by up-regulating Bax,Bad genes and down-regulating Bcl-2 and Bcl-xl genes.In addition,we performed transcriptomic sequencing analysis on A549 cells before and after phycocyanin treatment.8998 genes were significantly up-regulated and 8979 genes were significantly down-regulated by expression analysis.The GO and KEGG pathways results showed that there was a significant difference in the NF-kB pathway.Key difference genes such as THEM4,RIPK1,and TIRAP enriched for the kB pathway were verified at the transcriptional level.Finally,we examined the key proteins of the NF-kB pathway using western-blot and found that phycocyanin can Significantly inhibit the activity of NF-kB pathway.In the present study,We found that phycocyanin can significantly inhibit the growth and migration of non-small cell lung cancer cells in vitro and promote cell apoptosis by vitro cell phenotypic analysis.We used high-throughput sequencing analysis of transcriptomes to find that phycocyanin is involved in the regulation of multiple signaling pathways in cells.Among them,modulating NF-kB signaling pathways may have an important regulatory effect on lung cancer cell growth inhibition.It provides the necessary theoretical basis for the development of new anti-cancer functional food colorants and for the safe treatment of non-small cell lung cancer.