Tcr V β Gene Expression Profile Analysis Of Non-small Cell Lung Cancer Patients In Middle-aged Male

Objective:To discuss the expression and significance of TCR V β gene expression of non-small cell lung cancer patients in middle-aged male.Methods:1、Separating peripheral blood T cells of both healthy people and patients.2、Recording all the family of TCR Vβ expression patterns using flow cytometry3、Statisticing and analyzing the characteristics of all the family of TCR Vβ expression patterns.Results:1、The expression of peripheral blood TCR Vβ genes are high partly in middle-aged male with non-small cell lung cancer then normal control group’s.And CD8+TCR Vβ3、4and9expression have statistical significantce(P<0.05).2、The expression of CD8+TCRV β3and V β4have relevance (r=0.541, p=0.008).Conclusion1、The expression of peripheral blood TCR Vβ genes in normal middle-aged male can be no obvious specificity.2、The expression of peripheral blood CD8+TCR Vβ genes in middle-aged male with non-small cell lung cancer have obvious specificity. We speculate that there are some contact between the expression of peripheral blood CD8+TCR Vβ genes and tumor table.3、In peripheral blood, the expression of CD8+TCR Vβ genes in middle-aged male with non-small cell lung cancer showed significantly more specific then CD4+TCR V β’s.4、The expression of CD8+TCR V β3and V β4have homonymous relationship,showing the coupling phenomenon within the two genes.