Basic And Clinical Research On Chinese Medical Syndrome Of Lung Cancer

Primary pulmonary cancer which can simply called lung, is the most common malignant tumor. However there is no good effective treatment whether in Chinese medicine or western medicine. The main reason in Traditional Chinese Medicine theory, the concept of pulmonary cancer is usually confused, it is always just simply listed in numerous names in the past, such as Pulmonary mass, Hypochondriac lump, Cough, Hemoptysis, Blood spuyum, Chest pain, Fever. The author comes up with a new viewpoint that the symptom of Consumptive is basically accord with lung cancer. In this research explore and prove that cough, laryngopharyngeal paresthesia, ptyalism, short breath, wheezing sound, hemoptysis, blood sputum, chest pain, fever and other clinical manifestations in consumptive lung disease are similar to lung cancer. Lung cancer can be divided into the following 9 types: deficiency of both qi and Yin, stagnation of phlegm in the lungs, cold deficiency in the lungs, stagnation of phlegm in the interior, accumulation of phlegm-heat, disorder of qi, Yang deficiency due to phlegm stagnation, impairment of the descending and purifying effects of the lung, exterior heat and interior cold, upward adverse flow of the qi, phlegm retention due to consumptive lung disease, weak body resistance resulting in existence of pathogenic factors, deficiency of both the lung and the kidney, regulation disorder, deficiency of both qi and blood, deficiency of both Yin and Yang patterns and so other forms. And formulate treating methods and kepresentative prescription for every syndrome, especially deficiency of both qi and Yin, stagnation of phlegm in the lungs, and phlegm retention due to consumptive lung disease, weak body resistance resulting in existence of pathogenic factors. The two types of phlegm retention due to consumptive lung disease, weak body resistance resulting in existence of pathogenic and deficiency of both qi and yin, stagnation of phlegm in the lungs, whose kepresentative prescription were Runfeisanjie Decoction and Tinglixieshui Decoction which were made from classical medical books combined with clinical experience by writer.Through the clinical observation on 235 cases primary brouchogenic caranoma of lung, we can definitely describe the lung cancer. The type of deficiency of both qi and Yin, stagnation of phlegm in the lungs constitute 30.64% in the whole that have marked differences with other 8 types. P<0.01, which prove that primary brouchogenic caranoma of lung is the most common type. According to the appearance rate, also have the type of accumulation of phlegm-heat, disorder of qi, pertinatious phlegm stagnation, impairment of the descending and purifying effects of the lung, deficiency of both qi and blood, deficiency of both Yin and Yang, phlegm retention due to consumptive lung disease, weak body resistance resulting in existence of pathogenic factors, deficiency of both the lung and the kidney, regulation disorder, cold deficiency in the lungs, stagnation of phlegm in the interior, exterior heat and interior cold, upward adverse flow of the qi, phlegm retention due to consumptive lung disease. As for course of disease, the type of deficiency of both qi and Yin, stagnation of phlegm in the lungs from the beginning to the end, but deficiency of both qi and blood, deficiency of both Yin and Yang, deficiency of both the lung and the kidney, regulation disorder, cold deficiency in the lungs, stagnation of phlegm in the interior were centralized in 3 and 4 period, pointed out healthy qi consumed because of long disease, deficiency of Yin affecting Yang also have clinical leading meaning. There are 22 cases of phlegm retention due to consumptive lung disease, weak body resistance resulting in existence of pathogenic factors in the lung cancer. 19 cases adenocarcinoma constitute 86.36%, higher than squamous carcinoma 13.64%. We should focus on strengthening the spleen and promoting the dispersing functions of the lung, inducing diuresis and resolving dampness if pa thological reported the lung cancer patient caused by adenocarcinoma.But the systematic observation on 72 cases with the type of deficiency of both qi and Yin, stagnation of phlegm in the small cell lung cancer (SCCL), which proved that Runfeisanjie Decoction merged chemotherapy have reliable clinical curative effect, principle syndrome improvement rate high than control group P<0.01.Focus also shrinked, there is no greatly differences with remission rate (CR+PR) between treating group(55.55%) and control group (50.00%) after treatment (P>0.05),but (CR+PR+NC) treating group (91.66%) higher than control group (72.22%) (P<0.05), even from the quality of life improvement and stabilization aspects (P<0.05); as for toxic and side reaction rate, treating group lower than control group that have greatly differences<0.01. In the period of finishing chemotherapy, treating group higher than control group with survive rate in 1 year,2year,6 months. So, Runfeisanjie Decoction plus chemotherapy is an effective method for treating the type of deficiency of both qi and Yin, stagnation of phlegm in the lungs non-small cellular.To observe the effect of Runfeisanjie Capsule on inhibiting mouse Lewis Lung Carcinoma tumor growth. A piece of Lewis Lung Carcinoma was transplanted into the subcutaneous space on the armpit of each C57BL mouse. Mice were random separated into several groups. After orally administered, the mean weight of the subcutaneous tumors was examined, then the average tumor control rate was analysed. And observed the change of organization of tumor by light microscope and electron microscope, Bcl-2 and Bax were detected by the method of immunity histochemistry and the proportion of T lymphocyte were calculated by flow cytometer FCM. In the group treated by RFSJC, the tumor control rate was 36.5%, 35.0% and 30.1% respectively. Compared to the tumor model group, the weight and the volume of tumor were decreased significantly (P<0.01). Cells of inflamed infiltration in HE dyeing and hemorrhage band, a piece of necrosis zone, blood vessel less, apoptotic body appeared in electron microscope. The express of Bcl-2 and the value of IODT decreased greatly (P<0.01). The express of Bax changed little. The proportion of CD3+, CD4+ and CD8+ increased greatly (P<0.01). Being proved these results suggest that RFSJC could inhibit the growth of mouse Lewis Lung Carcinoma, and its mechanism may be related with its action of inducing the apoptosis of tumor cells .To investigate the effect and the mechanism of Runfeisanjie Capsule (RFSJC) on Lewis pulmonary tumor in vitro, we treated the Lewis cells with RFSJC plasma, the activation of anticancer were examined by MTT assay. The rate of inhibition and life prolongation were calculated and pathological sections were observed. Apoptosis of tumor cells was detected by flow cytometry and immune histochemistry after being treated with RFSJC. RFSJC inhibited Lewis cells proliferation significantly at the concentration above 16μg/mL for 72 h and its IC50 was 0.5μg/mL. The shape of cells appeared typical characteristic of apoptosis. The percentage of apoptosis increased significantly treated by RFSJC. After intervene by RFSJC 36hour later, the apoptotic percentage of Lewis lung cancer higher than control group in S period, (P<0.05), especially the group of 4.5ug/mL, S period of Lewis lung cancer amount to 40.1%, while cells number decreased in G0/G1 period as well as M/G2 period which compared to processing Lewis lung cancer, especially middle dosage group, proportion in M/G2 period was very low. It was thus evident that proportion of positive expression for p53 and Bcl-2 decreased obviously after medication, and the proportion of c-myc increased obviously (P<0.01) and color darkened. Result: RFSJC could inhibit the growth of pulmonary tumor cultured in vitro and it's antitumor mechanism may be explained that the cancer cells in S period of is accumulate show that the key link may be the blocking of the DNA synthesis.