Establishment And Evaluation Of Anger-out, Anger-in Emotion And Induced Combination Of PMS Diseases And Symptoms Rhesus Monkey Models

Objective:To complete the establishment and evaluation of anger-out,anger-in emotion and induced PMS Liver-qi invasion,Liver-qi depression rhesus monkey models,and then provide an appropriate platform for studing pathogenic mechanism of anger-out and anger-in emotion; After detection of neurotransmitters in central and serum, pathogenesis of emotional syndromes induced by anger-out and anger-in emotion maybe initially revealed;In order to provide valuable clues for clarifying Jingqianping and Jingqianshu granule'actional mechanism,we intervened PMS model monkeys with these two drugs.Methods: Experimental macaques were induced to show up emotional reaction with social level stress method,then anger-in,anger-out emotion macaques were screened by Emotional Evaluation Scale about Female Experiment Macaque scoring together with facial expression pattern recognition system,based on mentioned above experimental macaques were induced to show up PMS Liver-qi invasion and Liver-qi depression appearance combined with timing extrusion modeling,soon after their facial expression and behavior was secondary evaluated by scale scoring and pattern recognition, eventually Traditional Chinese Medicine Jingqianping and Jingqianshu Granule were respectively used to interfere.Serum of different periods were collected,then it was detected with different brain regions of norepinephrine,dopamine,5- hydroxytryptamine andγ- Aminobutyric Acid(only in brain regions) content,then the results combined with scale scoring were used to evaluate the models and to verify the effects of modeling.Results: The angry factor scores of anger-in,anger-out rhesus monkeys were significantly higher than the control(P<0.01),anger-out group higer than anger-in group(P<0.05).The depression factor scores of anger-in rhesus monkeys were notably higer than the control and the anger-out(P<0.01), Jingqianping and Jingqianshu granule respectively lower the angry and depression factor scores of PMS Liver-qi invasion and Liver-qi depression rhesus monkeys(P<0.05).Contrary to the control and emotional reaction group,the monoamine neurotransmitters content of PMS liver-qi invasion and Liver-qi depression rhesus monkeys increased substantially(P<0.05, P<0.01),then decreased to a large extent administrated with Jingqianping and Jingqianshu granule(P<0.05, P<0.01).Compared with the control group, NE and DA content in hypothalamus,NE,DA and 5-HT content in parietal cortex of PMS Liver-qi invasion group was significantly higher(P<0.05, P<0.01),NE and GABA content in pituitary was lower(P<0.05, P<0.01); DA and GABA content in pituitary,NE,DA and 5-HT content in hypothalamus and parietal cortex,GABA content in frontal cortex increased significantly(P<0.05, P<0.01),GABA content in pituitary, hypothalamus and frontal cortex lowerd(P<0.05, P<0.01).Conclusions:Rhesus monkeys could be induced to show anger-out and anger-in emotion with social level stress method, subjective observation, scale scoring and neurotransmitters detection could properly quantitate anger-out,anger-in emotion and symptoms of the disease.Anger-out and anger-in emotion had an relationship with the change of monoamine neurotransmitters content in serum , pathogenesis of PMS Liver-qi invasion and Liver-qi depression was closely related to monoamine neurotransmitters in serum and brain and GABA in brain.The regulation of neurotransmitters in serum and brain of Jingqianping and Jingqianshu Granule may be the mechanisms of treating PMS Liver-qi invasion and Liver-qi depression.