Expressions Of 5-HTR2C In Different Brain Regions Of The Model Rats With Anger-out Or Anger-in And Effects Of Liver-regulating Compound Recipe

Objective:Our aims are to study the mechanisms of anger emotion and the Liver-regulating Compound Recipe ( Jingqianping granule and Jingqianshu granule)acting on the anger-in and anger-out emotions,by investigating the expressions of 5-hydroxytryptamine 2C receptor(5-HTR2C) in different brain regions of anger-out or anger-in model rats,and the effects of Liver-regulating Compound Recipe ( Jingqianping granule and Jingqianshu granule)on the expression.Methods:Model rats with anger-out or anger-in emotional reaction are induced by social isolation plus resident intruder stress, evaluated by open-field test and aggressive behavior test,and some of them were intervened with Jingqianping(Jingqianshu)granule. After introducing model,we killed the rats by decapitation,dissected different brain regions(prefrontal cortex, parietal cortex, hippocampus and hypothalamus)。Then we analyzed the expressions of 5-HTR2C in partietal cortex,frontal cortex,hypothalamus and hippocampus of the three groups (including normal control group, anger-out or anger-in model group, Jingqianshu group or Jingqianping group) by the methods of RT-PCR and Western blot.Results: Compared with the normal control group,the mRNA and protein expressions of 5-HTR2C incressed obviously (P<0.01) in frontal cortex and hippocampus,decreased obviously (P<0.01) in hypothalamus of anger-out model rats.The mRNA and protein expressions of 5-HTR2C decreased obviously (P<0.01) in partietal cortex,frontal cortex and hippocampus, incressed obviously (P<0.01) in hypothalamus of the Jingqianping group compared with the anger-out model group.Compared with the normal control group,the mRNA and protein expressions of 5-HTR2C incressed obviously (P<0.01) in hippocampus,decreased obviously (P<0.01) in hypothalamus,and had no obviously changed in frontal cortex of anger-in model rats.The mRNA and protein expressions of 5-HTR2C decreased obviously (P<0.01) in hippocampus,and had no obviously change in frontal cortex of the Jingqianshu group compared with the anger-in model group.Conclusion:The expressions of 5-HTR2C in different brain regions were closely related to anger-out and anger-in of rats.The changes of the expressions may be one of the main reasons to induce rats anger-out or anger-in.The expressions of 5-HTR2C changed obviously in anger-out and anger-in model rats by using Liver-regulating Compound Recipe,so the regulating of Liver-regulating Compound Recipe to rats in different regions may be one of the mechanisms effecting the emotion of the anger-in or anger-out rats.