| Purpose:This experiment through research the animal model of the syndrome of Dampness Incumbering Middle Energizer to aquaporin gastrointestinal differentially expressed and the study on the control mechanism of Pingwei Dosage, For Pingwei Dosage fluid electrolyte scattered regulation of opening a new way, So Pingwei Dosage on the body of the mechanism of the effect of scattered had more system, further more comprehensive overview of repeal.Methods:Using the HuangXiuShen etc comprehensive made mode methods are made moulds. By immunohistochemical method determined AQP1,AQP2,AQP3,AQP4,AQP5,AQP7,AQP9Distribution expression in stomach to Understand mould and give medicine made after the water electrolyte metabolic generate differences mechanism. By measuring the AVP, VIP, serum levels of rats in vivo to understand the changes of water electrolyte metabolism. By measuring in stomach tissue and mucous membrane AQP1, AQP9, VIP, cAMP, AC, PKC, PI3K PKA, to illustrate the content change made mold and give medicine rat hormone levels after the change and adjust the cell water electrolyte in various channels protein and related signal transduction pathways signal molecular changes. Thus come to know of moisture resistance flat stomach scattered the mechanism of action of the b.f card model.Result:1 The distribution of Seven aquaporin in the stomach1.1 The distribution of Seven aquaporin In the cardia of stomach1.1.1 AQP1 The distribution of normal rats AQP1 mainly distributed in mucosal layers, After the made in its upper die increased, the distribution of mucosal, After the drug in cardia mucosa distribution is more natural recovery group and the model group has decreased comparison.1.1.2 AQP2 In the distribution of cardia AQP2 mainly distributed in the mucous membrane layer also. The modes and drug maker, Its distribution no obvious difference.1.1.3 AQP3 The modes and drug maker, The distribution of AQP3 than in cardia mucous membrane blank group increased。Its distribution reduced after treatment of pingwei Dosage.1.1.4 AQP4 In the distribution of cardia AQP4 mainly distributed in mucosal layers, Model group distribution is blank group increased, pingwei dosagegroup more natural recovery group reduced,The distribution of blank group with no difference.1.1.5 AQP5 AQP5 mainly distributed in cardia mucosal layers, Model group compared with blank group. The division has increased, pingwei dosage group scattered group distribution is more model group reduced.1.1.6 AQP7 Blank group mainly distributed in the upper AQP7 the mucous membrane layer, The modes and give medicine made after the AQP7 in cardia mucous membrane layer distribution increase. Among them, the biggest increase model group.1.1.7 AQP9 AQP9 blank group mainly distributed in the upper layer, mucosal, after The modes and give medicine made, Model group and natural recovery group of distribution reduction, pingwei dosage group returned to blank group level.1.2 the distribution of aquaporin in the stomach1.2.1 AQP1 AQP1 in the blank group, mainly distributed in the stomach mucosa layer. he modes and give medicine made a blank, model group after group distribution increase, And natural recovery group based on the model group, continued to increase, Flat stomach scattered group and blank group no difference.1.2.2 AQP2 AQP2 in the blank group, mainly distributed in the stomach mucosa layer. The modes and give medicine made after the distribution is more blank group AQP2 significantly reduced, natural recovery group of basic don't express, flat stomach scattered group and the blank of AQP2 in treatment group than in the blank group distribution significantly increased.1.2.3 AQP3 AQP3 in the blank group, mainly distributed in the stomach mucosa layer, the mould, model group made peace AQP3 distribution of stomach scattered group increased.1.2.4 AQP4 AQP4 in the blank group, mainly distributed in the stomach mucosa layer, the mould, model group made AQP4 distribution increase, flat stomach scattered group and blank AQP4 distribution in treatment group of reduction.1.2.5 AQP5 AQP5 in the blank group, mainly distributed in the stomach mucosa layer, the mould, model group made after natural recovery group distribution and increased, whereas flat stomach scattered group and blank in treatment group and blank group. No difference.1.2.6 AQP7 AQP7 in the blank group, mainly distributed in the stomach mucosa layer, the mould, model group made after natural recovery increased, but the distribution of distribution is reduced, flat stomach scattered group and blank in treatment group distribution increase.1.2.7 AQP9 AQP9 in the blank group, mainly distributed in the stomach mucosa layer, the modes and give medicine made, flat stomach after scattered group distribution enhanced.2 Water channel protein adjustment mechanism of relevant factors in the determination2.1 Long the adjustment mechanism is determined2.1.1 AQP1 and AQP9 in gastric content is determined2.1.1.1 AQP1 AQP1 model group in the content and blank cardia organization, is decreased; group compared Natural recovery group peace with empty stomach scattered group group compared AQP1 content low, lower than the model group; Flat stomach AQP1 scattered group of content is natural recovery group of high. Model group AQP1 among stomach mucosa group compared with the content, no difference blank; Natural recovery group, compared with blank group AQP1 content decreased. Flat stomach scattered group and blank give medicine group, compared with model group are increasing its content.2.1.1.2 AQP9 after the natural recovery group based on the model group, are still falling natural recovery group and flat stomach, AQP9 scattered groups like comparing the content low. Model group in the stomach AQP9 organized group compared with blank content, there was no significant change; Other each group compared with blank, also have no apparent change.2.1.2 Hormone adjustment mechanism is determined2.1.2.1 Serum VIP Model group the VIP content and blank group compared not decline, no obvious difference, after three days, the natural recovery group based on the model group, is still falling; Flat stomach contents of scattered group VIP higher than the natural recovery group.2.1.2.2 Serum AVP Model group the VIP content and blank group compared not decline, no obvious difference, after three days, the natural recovery group based on the model group, is still falling; Flat stomach contents of scattered group VIP higher than the natural recovery group.2.1.2.3 Tissue VIP Model group in the stomach tissue VIP group compared with blank content decreased, after three days later, the group of natural recovery increased, but the content of VIP below blank group; Blank in treatment group peace with natural recovery stomach scattered group, content increased compared group.2.2 The determination of short-term adjustment mechanism2.2.1 The stomach the determination of VIP hormone adjustment mechanism2.2.1.1cAMP Model group in the cAMP in the stomach mucosa, compared with blank group content with a certain degree of lower, but no significant difference; Natural recovery group based on the model group, continue to reduce; Flat stomach scattered group and blank in treatment group than the content of natural recovery group increased cAMP. The stomach in the cAMP, the organization variation in content, but no significant difference in building mode, model content is blank group fell.2.2.1.2 AC Model group AC among stomach mucosa group compared with blank the content, no difference; But natural recovery group, compared with blank group the content is decreased; AC Blank in treatment group peace with natural recovery stomach scattered group, compared to the content of the AC significantly increased. Model group AC among stomach organization's content is blank group has dropped, after three days, the natural recovery group, blank give medicine group stomach contents of peace are scattered group has risen, and flat stomach scattered group and the content of blank in treatment group than natural recovery group of content.2.2.1.3 PKA Model group in the stomach mucosa PKA group compared with blank the content, there was no significant difference, but the content of natural recovery group than model group and blank all has different rate reduce; Blank in treatment group peace with natural recovery of stomach scattered PKA group compared the content rise. Model group PKA of content is blank group decreased, but not significant; After three days, the natural recovery group, flat stomach scattered groups and blank in the treatment group was relatively model group PKA are increasing, but this was not statistically significant.2.2.1.4PKC Model group in the stomach mucosa PKC group compared with blank the content, declined, and after three days, the content of natural recovery group in lower, but still there are no statistical significance. Model group PKC among stomach tissue, compared with other group content of no significant differences.2.2.1.5 PI3K Model group in the stomach PI3K organized group compared with blank content, after three days, the decline in the content of natural recovery still in model group level; Blank in treatment group peace with natural recovery stomach scattered group, PI3K group compared the content increased.Great summaryMoisture resistance modes and the b.f card model made after the treatment, not only affect the distribution of water channel protein stomach the change, but also caused the water channel protein stomach content change, the most main is a cause of gastric mucous membrane layer distribution and the water channel protein content of change. Explain this model and flat stomach for water channel scattered through affecting content and distribution change the protein to cause a change of water electrolyte in mice. Around the hormones such as water channel protein AVP, VIP and signal transduction pathways in various signal molecules such as cAMP, AC, PI3K PKA, PKC, also produced the corresponding change. Instructions for rats scattered flat stomach inside the influence is through water electrolyte water channel protein and these hormones and signal molecular mechanism of action to achieve. |
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