Automated Chemiluminescence Immunoassay System

Immunodiagnostic technology is one of the most significant methods applied in the modern clinical lab and life-scientific research. The Immunodiagnostic is the powerful tool to diagnose such diseases as the tumor, diabetes, reproductive, and thyroid gland, etc. Traditional radioactive immunoassay is gradually replaced by new emerged chemiluminescence immunoassay technology in the latest decade years due to its complicated handwork procedure, long reaction time (up to 48 hours), disputed environment pollution. The full-automatic instrument design involves many high-tech and in structure-complicated fields like electrons, machinery, optics, the technology of the computer, biochemistry, etc., otherwise, enormous research and development expenses will have to be considered in the earlier stage. The research of domestic relevant products is still at the starting stage at present.This paper consults foreign relevant products and technology, aims at the current domestic situation, proposes the integrity design of the chemiluminescence immunoassay. Two key modules, the pipetting system and the temperature control system, are concretely designed and realized.The system reaction principle: Regards alkaline phosphates enzyme conjugated to polyclonal antibody or antigen as the liquid phase carrier, the antigen or antibody coated plastic pearl as the solid carrier, the substrate (AMPPD ) as the chemiluminescence materials, to run the clinical assays.The chemiluminescence system is mainly made of following subsystem: The reaction tube conveyance system;test unit loading system;the sample loading system;the bar code scanning system;the reagent loading system;pipetting system;wash system;chemiluminescence counting system, computer control system.The pipetting system adopts the control chip-L297 and the electric current driver chip-L6202 to drive the step motors, other than applying the complicated program control used with DSP(Digital Signal Processing), the pipeline that is linked to the pipetting system adopts the electromagnetic valve to control the direction of the fluid, the pipetting system can accomplish the pipetting and washing procedure in one cycle;The 18-bits high precision A/D chip MAX132 is used in the temperature collecting, the PID (Proportional-Integral-Differential ) algorithm is applied to the temperature control system, the temperature control precision can be up to 0.05 ℃.