The Method Of Chemiluminescence Based On Nanoparticles-labled Probe

The emergence of nanotechnology is opening a new horizon for biological detection, and offering an excellent prospect for highly sensitive assays of biomolecules.In recent years, several new ultrasensitive detection methods for biomolecules have been developed based on metal nanomaterials.In this paper, based on nano-materials labeled probe in chemiluminescence applications,we have some research as follows:(1). horseradish peroxidase (HRP) was assembled onto MWCNTs templates layer-by-layer (LBL) through electrostatic interactions with polyion PDDA, and further conjugated with AFP secondary antibodies (Ab2) as the enzyme label.Thereby increasing the number of enzyme label, combined with magnetic separation technology,under the optimum conditions of luminol-H2O2-HRP-BPB CL system and the sandwich immunoreactions detection of AFP in human serum, a linear range from 0.02 to 2.0 ng/mL (R=0.9980) was obtained with the detection limit of 8.0 pg/mL.The results show that the method is higher sensitivity and wider linear range than the direct method of adding labeled antibody. In actual clinical testing and other tumor markers AFP has broad application prospects.(2). A simple and sensitive method was developed for the detection of DNA hybridization based on FI-CL and signal amplification by bio-bar-code functionalized magnetic nanoparticle label. To achieve the quantitative detection and selective identification of target DNA detection of a specific DNA sequence,a linear range from1.0×10-15 to1.0×10-14 M was obtained with the detection limit of 0.32 fM for the target DNA. The method has the advantages of simple apparatus, high sensitivity, rapid detection etc, and provides a theoretical basis for early diagnosis of disease.