| Microfluidics is a discipline that studies and manipulates tiny fluids.It uses microfluidic chips as carriers to complete various biochemical experiments,and has been widely developed and applied in the fields of biology and chemistry.As an important branch of microfluidic chips,open-channel microfluidic chips have received more and more attention in recent years.Compared with traditional microfluidic chips,their preparation process does not require bonding and has higher flow channel accessibility,fluid spontaneous drive and other advantages make it have great development potential.However,there are relatively few studies on open-channel microfluidic chips,and the technological development route is rather vague.Therefore,this paper will study the design and fabrication technology of open-channel microfluidic chips.Starting from the principle of spontaneous flow of fluid in the open channel,using CO2 laser ablation as the process basis,combined with the surface modification method,an open channel microfluidic chip based on PMMA and agarose gel was designed and fabricated.The prepared microfluidic chip was functionally verified.The main research contents are as follows:(1)Explore the application principle of spontaneous capillary flow in the field of open channel microfluidic chips,study the fluid flow laws in open channels with rectangular and V-shaped cross-sections,and discuss the factors that affect spontaneous flow.According to the law of spontaneous fluid flow in the open channel,the functionalized open channel structure is explored to provide a basic theoretical basis for the subsequent preparation of open channel microfluidic chips.(2)Using PMMA as the chip substrate and CO2 laser ablation as the processing method of the microfluidic channel,experiments were carried out to obtain the preparation process parameters of the specific microstructure.Two kinds of surface hydrophilic modification methods based on PVA/Lb L were experimentally studied respectively,and the most optimized preparation method of open channel microfluidic chip based on PMMA material was formed.Finally,the preparation of the functionalized open channel microfluidic chip was completed,and the experimental verification was carried out.(3)Using the hydrophilic material agarose gel as the substrate,the preparation technology of the open channel microfluidic chip was studied,and the performance of the agarose gel microfluidic chip was verified by experiments.Finally,according to the deformation characteristics of agarose gel caused by water evaporation,the storage method of agarose gel open channel microfluidic chip was explored.(4)In order to further verify the feasibility of the open channel microfluidic chip design and fabrication method proposed in this paper,the application of copper ion concentration detection was carried out using the prepared microfluidic chip.The concentration-gray value curve equation was obtained by curve fitting through the copper ion color reaction combined with the image processing method,and the low-cost and rapid detection of copper ion concentration based on the open channel microfluidic chip was realized. |
