Effect Of Blood Flow Restriction Combined With Intermittent Running On Children’s Growth And Development

Research purposes:The over emphasis of social media on the importance of appearance has led to many parents ’anxiety about their children’ s heiGht.More and more children in growth and development stage are receiving the intervention of secreted recombinant human growth hormone(rhGh).however,inappropriate use of rhGh will increase the risk of cardioVAScular disease,type 2 diabetes and cancer among users.Exercise is often regarded as a common means to promote bone development in children,but specific exercise prescriptions for promoting growth related hormone secretion and bone development in developing children are rare in experimental studies.Physical exercise can significantly promote hormone secretion and bone remodeling in adults under blood flow restriction,but the positive effects of this exercise intervention on children ’s growth and development are poorly studied.This study hopes to conduct prospective experimental research on this issue,so as to provide theoretical and practical references for sports intervention to promote children’s growth and development.Research Methods:This study selected 36 children from an experimental primary school in Chongqing as the experimental subjects.The trial was a randomized,crossover,single blind design.During the entire experimental process,the subjects entered the laboratory four times for relevant testing interventions.The first test:blood collection and measurement of children’s silent arterial occlusive pressure(AOP)test to determine the BFR protocol.After that,conduct a maximum oxygen uptake test to familiarize yourself with the use of the scoring scale and exercise plan.The second test:Perform the second blood sampling,familiarize yourself with the blood flow limiting device,and test the maximum oxygen uptake(VO2 max)under blood flow limiting conditions.Finally,rehearse the entire process of the experiment twice.The third and fourth tests were formal experiments.The subjects were divided into Group A(n=18)and Group B(n=18)using a random number table method,with 18 people in each group.Group A subjects performed the exercise matched blood flow restriction(BFR)protocol on the third test.After an interval of 1 week,the exercise matched placebo(PBO)protocol was performed on the fourth test(only the compression band was bound,without compression).Group B subjects performed the exercise matched PBO protocol on the third test.After an interval of 1 week,the exercise matched BFR protocol was performed on the fourth test.A formal experiment includes three stages.The first stage is to perform aerobic running+8 warm-up exercises with a heart rate of≥140 bpm(10 min).In the second stage,exercise intervention was conducted,with intermittent running on a treadmill at 70%VO2max intensity,and BFR or PBO treatment(60 min)was performed according to the protocol.In the third stage,post intervention testing was conducted,including blood sampling,RPE and VAS testing at 3 0,60,90,120,and 24 hours after exercise.SPSS22.0 software and GraphPad Prism 9.0 were used to process the data before and after the experiment.Research results:(1)After BFR treatment,the serum levels of growth hormone(Gh),insulin-like growth factor 1(IGF-1),and insulin-like growth factor binding protein 3(IGFBP-3)were significantly hiGher than the serum hormone level after PBO treatment at the 30th,60th,90th,120th,and 24th min(P<0.05),and the serum growth related hormone levels were significantly hiGher than the baseline level at the corresponding time points(P<0.05).(2)There was no significant difference(P>0.05)in the levels of type I amino terminal propeptide(PINP)and osteocalcin(OC),the markers of bone formation,between the subjects treated with BFR and the PBO group at the 3 0th,60th,90th,120th,and 24th minutes after BFR treatment,but there was a significant difference(P<0.05)when they were hiGher than the baseline level at the 6 0th,90th,120th,and 24th hours after BFR treatment.At the 3 0th,60th,90th,and 120th min after BFR treatment,type I collagen crosslinked C-terminal peptide,a bone resorption marker,was detected in subjects(β-The level of CTx had no significant change compared to the PBO treatment group(P>0.05),but at the 24th hour after treatment,it was significantly lower than the PBO treatment group and the baseline level(P<0.05).(3)The serum interleukin 6(IL-6)level of the subjects after BFR treatment was hiGher than that of the control group at the 6 0 th and 9 0 th min,and significantly hiGher than the baseline serum IL-6 level at the 30th,60th,90th,and 120th min(P<0.0 5).however,there was no significant difference between the PBO treatment group and the baseline level at the 2 4 th hour after BFR treatment.(4)There was no significant difference in serum cortisol levels between subjects treated with BFR and PBO at the 30th,60th,90th,120th,and 24th minutes after BFR treatment,and there was no significant change in serum cortisol levels at the 24th hour after BFR treatment(P>0.05).however,serum cortisol levels were significantly hiGher than baseline serum cortisol levels at the 30th,60th,90th,and 120th minutes after BFR treatment(P<0.0 5).(5)The serum lactic acid level of the subjects after BFR treatment was significantly hiGher than that of the PBO treatment group at the 3 0 th,60 th,and 90 th min,and the serum lactic acid level was significantly hiGher than the baseline serum lactic acid level at the 30th,60th,90th,and 120th min(P.(6)The serum creatine kinase(CK)level of subjects treated with BFR was significantly hiGher than that of PBO at the 3 0th,60th,90th,and 120th min after exercise,and the serum CK level was hiGher than the baseline level of serum CK at the 30th,60th,90th,120th,and 24h after exercise(P<0.0 5);however,there was no significant difference in serum CK levels between the BFR treated group and the PBO treated group at the 24 th hour after BFR treatment(P>0.05).(7)Subjects ’subjective fatigue sensation score(RPE)was significantly hiGher than the baseline RPE score at the 30th,60th,and 90th minutes after BFR treatment(P.(8)After BFR treatment,the level of visual simulation score(VAS)in subjects was significantly hiGher than that in PBO treatment group at the 3 0 th,6 0 th,and 9 0 th min,and the VAS score was significantly hiGher than the baseline level of VAS score at the 3 0th,60th,90th,and 120th min after exercise(P<0.05);however,at 24 hours after exercise,there was no significant difference in VAS scores between the PBO treatment group and the baseline level(P>0.05).Research conclusions:(1)After a single blood flow restriction combined with intermittent running,the concentration of growth and development related hormone indicators in the group without blood flow restriction was significantly hiGher than that in the group without blood flow restriction;This indicates that blood flow restriction combined with intermittent running can more effectively stimulate growth and development related hormone activity.(2)After a single blood flow restriction combined with interval running exercise,the markers of bone formation in children significantly increased while the markers of bone absorption showed a downward trend.BFR combined with interval running exercise can stimulate bone formation and intensify the effect.(3)After a single blood flow restriction combined with interval running,the blood lactic acid,serum cortisol,VAS score,and RPE score showed a trend of first increasing and then decreasing,and there was no significant difference in inflammatory indicators CK and IL-6 between the experimental group and the control group 24 hours after exercise,indicating that the exercise load was within the acceptable range of the subjects and did not increase muscle microdamage.