| Objective: To investigate the characteristics of superoxide dismutase(SOD),inducible nitric oxide synthase(iNOS),and blood glucose changes in pancreatic tissue of rats with high-voltage electrical burn and the intervention effect of N-acetylcysteine(NAC).Methods: We randomly assigned 220 SD male rats to four groups: false injury group,electrical injury group,saline group,and NAC group.There were sixty animals in each group false injury group,and the electrical injury group.The average number was divided into six different time phases: 0h after electricity,8h after electricity,24 h after electricity,48 h after electricity,72 h after electricity,and 1W after electricity,ten rats in each phase;There were 50 animals in each group of the saline group and NAC group,and divided the average number into five different time phases: 8h after electricity,24 h after electricity,48 h after electricity,72 h after electricity and 1w after electricity,and ten rats in each phase.The mold-making process was completed by 3kV highvoltage electricity through rats’ left anterior and right hind limbs for 3s.Except for the fake injury group and the electrical injury group,rats in the other groups were injected with 100mg/kg NAC and the same volume of 0.9% Na Cl solution intraperitoneally for drugs and intervention controls.The serum of rats of each group of time phases was collected to detect amylase and calcium ions.The rat pancreatic tail was stained with HE to observe the pathological changes in the pancreas.Superoxide Dismutase(SOD)activity and inducible Nitric Oxide Synthase(iNOS)concentration were determined in pancreatic tissue.Lastly,statistics and analysis of the data obtained.Results: 1.HE staining showed regular morphology of pancreatic tissue cells,uniform size and compact arrangement of islet cells,and no vacuolation in the cytoplasm.Pancreatic tissue cell morphology was disordered,islet cell arrangement was loose,and cytoplasmic vacuolation was obvious.With the progression of the disease,the number of vacuolated cells increased.The pancreatic morphology of the treatment group was more regular than that of the electrical injury group and saline group,and the number of vacuolated cells was reduced.2.The blood glucose concentration of rats in the electro-trauma group was significantly higher than that in the pseudo-injury group(P < 0.05)at all times after injury,and the blood glucose concentration in rats had double peaks on the third and fifth days after injury,and gradually decreased on the 6-7th day.There was no significant difference in blood glucose concentration between the NAC group and the saline group(P > 0.05),but blood glucose decreased in the saline group.3.The iNOS content of pancreatic tissue in rats in the electrical injury group was significantly higher than that in the pseudo-injury group(P <0.05)at all times after injury,showing a trend of first decreasing and then increasing and finally decreasing,with a low peak at 24 h after injury and a peak at 72 h;compared with the saline group,the content of pancreatic tissue iNOS in rat pancreatic tissue in the NAC group did not change significantly(P > 0.05),but there was a trend of reduction.4.The SOD activity of pancreatic tissues in the electrical injury group was significantly lower than that in the pseudo-injury group(P < 0.05)at all times after injury;the SOD in the electrical injury group increased slowly from 0 to 24 h and gradually decreased from 24 h to 72 h.Compared with the saline group,SOD activity was significantly increased in the NAC group(P < 0.05).Conclusions:1.A high-voltage burn damaged rats’ pancreatic tissue,which showed vacuolar changes in islet cells.2.High voltage burn leads to increased blood glucose concentration in rats;3.High voltage burn results in increased iNOS content and decreased SOD activity in the pancreatic tissue of rats.4.NAC has a particular improvement effect on the pancreatic injury of high-voltage burn rats but has no significant effect on blood glucose concentration,iNOS content,and SOD activity in pancreatic tissue. |
