| Objective: High-voltage electric burn is a special wound, which cancause microcirculation disorders in the part and (or) body distant tissue andorgan regardless of the burn area size. The current mainly flows along thelow-resistance blood and nerve through the body, which causes extremevascular injury. For capillaries, the current directly damages the vascularendothelial cells and leads to a large number of vasoconstrictor substancesreleased by vascular and vasoactive substances. Therefore, that will cause aproinflammatory state and hypercoagulable state, increase microvascularpermeability and intravascular leukocyte-endothelial adhesion, result inerythrocyte aggregation and micro-thrombosis, and increase blood viscosity.The slow blood flow thereby affects the microcirculation blood flow, whichcauses the body microcirculation experiments. Experiments have provedthrombospondin (TSP) generate inhibition for vascular repair, and vonWillebrand factor (vWF) is one of the hallmarks of vascular endothelial celldamage. The objective of this experiment is to observe TSP, vWF affected byhigh-voltage current, further explore the mechanism of body’smicrocirculation disorder caused by damages of endothelial cells underhigh-voltage electric burn, and intervention of Ulinastatin (UTI) andmethylprednisolone (MP) affecting the expression of serum TSP and vWF,therefore, that will provide a experimental basis for the prevention andtreatment of microcirculation in high-voltage electrical injury.Methods:1Group:240healthy adult SD rats (the Experimental Animal Center ofHebei Medical University, and certification number1211057), male or female,are randomly divided into four groups, and60rats in each group. The groups are false high-voltage electrical burned group (control group), high-voltageelectrical burned experiment group (experiment group), high-voltage electricalburned UTI treatment group (referred to treatment group1), and high-voltageelectrical burned MP treatment group (referred to treatment group2). Thesefour groups are divided into electric shock before15min, electric shock after5min,1h,2h,4h,8h6different time phases, each phase10rats.2Preparation: Number and weigh the rats, then shed the rats’ feather ofleft upper limbs, right lower legs and the chest, and record them. Theexperimental drug is dubbed according to required concentration.3Duplicate the high-voltage electric burn model: Connect thetransformer and pressure regulator wire. Rats (40mg/kg) are anesthetized with1%sodium pentobarbital by means of intraperitoneal injection, after thesuccess of the anesthesia, put rats in special shock experiments on the stage,fixed limbs, respectively fix two1cm×1cm electrodes in the rat hair removalarea of left upper limbs (current entrance) and right lower limbs (currentexport). Switch on the power, adjust boost pressure regulator to output voltage2kV, connect the power of output, make high-voltage electric flow through rat,shock time is3s, the current strength of (1.85±0.25) A, the control groupoffers false electrical injury model that only connects even wires but noelectricity produce. Within5min of electrical burn, treatment group1isinjected UTI solution (5×104u/kg, is that7ml/kg administration) inabdominal cavity, treatment group2is injected MP solution (750mg/kg, forthat6ml/kg administration) in abdominal cavity, and the control group and theexperiment group are injected2mL/kg saline in abdominal cavity.4Collection and Detection: Operate the rat model replicated successful,open the chests, exposure heart, collect open-heart blood6ml, and let themrest for30min, put the serums in centrifuge after them precipitated,centrifuge them at3000r/min for10min, take clear liquid in Eppendorf tubeand preserve them in-70℃conditions.5Index Test: Apply ELISA double sandwich antibody method, test TSP,vWF serum content of each rats group for six phases. 6Analysis of Statistics: Apply SPSS13.0software and line two factorialdesign analysis of variance, and test p <0.05is of significance level.Results:1Changes of Serum TSP ContentThe TSP content of electrical injury group is higher than control group(main effect F=62.54, P <0.001); the time variables affect group’s TSPcontent after its injured,(F=3.52, P <0.01), that is, after injury of5min~8h,each phase is higher than the value of its group before injury (P <0.01), andreaches the highest value to4h after injury, which is (163.28±38.28) ng/mLhigher than the pre-injury (and108.91in1990±25.07) ng/mL, and then thevalue gradually fells, and remains at a high level.The TSP content of UTI treatment group is lower than electrical injurygroup (main effect F=23.06, P <0.001); after injury of5min，serum TSPcontent of treatment group1is still higher than the electric shock of15min,the difference is signifance (p <0.05); after injury of4h, TSP content of UTItreatment group basically returns to the level before the injury of15min, thereis no significant difference (p>0.05).The TSP content of MP treatment group is lower than electrical injurygroup (main effect F=171.49, P <0.001); treatment group2is affected bytime variables (main effect F=2.76, P <0.05), after injury of5min,1h,2h,4h,8h, the treatment group is significantly lower than the values in this groupbefore injury (P <0.01).2Changes of Serum vWF ContentThe vWF content of electrical injury group is higher than that in thecontrol group (main effect F=103.520, P <0.001); after injury, vWF contentof electrical injury group is affected by time variables (main effect F=5.205,P <0.001), after injury of5min~8h, each phase value is higher than thatbefore injury (P <0.001), immediately after injury, the value reaches thehighest value and then follows according to time, after injury of8hours, thevalue is still higher than the level.The vWF content of treatment group1is lower than the electrical injury group (main effect F=4.046, P <0.05), and it is affected by time variables(main effect F=7.453, P <0.001), after injury of5min~8h, each phase valueis higher than the value that before injury (P <0.001), and the value decreasesgradually according to time, after injury of8h it still remains at a high level.The vWF content of treatment group2is lower than the electrical injurygroup (main effect F=44.29, P <0.001); it is affected by time variables (maineffect F=4.73, P <0.01), after injured of5min,1h,2h,4h, each phase value ishigher than the pre-injury value (P <0.001), after treatment for8hvWF, thevalue basically returns to the level before electric shock.Conclusion:1The high-voltage burns can cause serum TSP content increase, andthe value reaches the highest level after injury of4h.2The high-voltage burns can cause serum vWF levels ascend and thevalue descend after injury of8h.3The high voltage produces TSP，vWF by stimulating the body, whichexplains that the current causes endothelial cell damage leading to the increaseof endothelial cells permeability, micro-thrombosis and blood viscosity, affectsthe microcirculation blood flow and body microcirculation.4UTI and MP inhibits blood TSP, vWF generation after high-voltageburned rats, therefore, it reduces the permeability of rats’ endothelial cellsafter electrical injury, mitigates the damage at the edema, and improvesmicrocirculation blood flow. |
PDF Full Text Request