Effect Of Phenylalanine On Symptom Improvement And Intestinal Flora In Rats With Constipation Induced By Loperamide

Objective:Functional constipation is a globally prevalent gastrointestinal functional disorder.Phenylalanine(Phe)is considered an important signaling molecule in the interaction between microbes and their host.Our previous research results indicate that changes occur in the gut microbiota composition of women of childbearing age with functional constipation,accompanied by a decrease in serum Phe levels and abnormalities in its metabolic pathways.Therefore,we conducted animal experiments to preliminarily explore the role of Phe in reshaping the gut microbiota composition and alleviating constipation.Methods:(1)Model construction: Loperamide(Lop)is used to induce a rat constipation model,with phenylalanine intervention.The experimental animals are randomly divided into three groups according to a random number table,namely the control group,the loperamide(Lop10 mg/kg)group,and the Lop+Phe group.(2)Evaluation of intestinal function: Intestinal motility is assessed by calculating the number of bowel movements in 24 hours,fecal water content,and carbon propulsion experiment.(3)Evaluation of gut microbiota composition: Fecal samples are collected for metagenomic sequencing to compare the changes in gut microbiota profiles among the three groups.Results:(1)Phe intervention significantly alleviated constipation symptoms in rats induced by Lop;(2)Phe intervention reshaped the gut microbiota composition of constipated rats induced by Lop.Conclusion:Phenylalanine may improve constipation symptoms in rats induced by Lop by reshaping the gut microbial community structure.