Effects Of Aflatoxin B1 On Neurobehavior In Zebrafish Based On Lipidomics

Aflatoxin contamination has been a key concern worldwide,with aflatoxin B1(AFB1)contamination being of high frequency and extremely toxic.Recent studies have found that aflatoxin has a serious damaging effect on the structure and function of brain tissue.However,the specific toxic effects and mechanisms of aflatoxin on brain tissues are still unclear.Therefore,in this study,a lipidomic approach was developed to investigate the disruption of lipid metabolism in brain tissue due to AFB1 in adult zebrafish,and to reveal its effects and significance on metabolic pathways,which also provides a basis for drug treatment,with the following main findings:1.The zebrafish were divided into three groups: control group,low dose group(5 μg/L)and high dose group(20 μg/L),and were exposed for 7 consecutive days.After the exposure,AFB1 was analyzed qualitatively and quantitatively in the brain tissue.The results showed that AFB1 was detected in the brain tissue of the low-and high-dose exposure groups,which were 1.558±0.142 μg/kg and 2.420±0.266 μg/kg,respectively.2.The effect of AFB1 on the neurobehavior of zebrafish was determined by behavioral analysis,and it was found that low concentration of AFB1 significantly promoted the movement of zebrafish,while high concentration of AFB1 inhibited its movement behavior.The trajectories of zebrafish showed a trend of marginalization,and the marginalization tended to be more serious as the exposure concentration increased.3.A lipidomics analysis method based on Waters Synapt ion mobility time-of-flight mass spectrometry(UPLC-IM-TOF-MS)was established.By matching with the self-built library,the analysis method was identified from zebrafish brain tissue 1734 kinds of lipid compounds,a total of 87 subclasses.4.By performing lipidomic analysis of zebrafish brain tissues after AFB1 exposure to obtain the resulting 114 differential lipids,significant metabolic changes including fatty acids(FA),glycerolipids(GL),glycerophospholipids(GP)and sphingolipids(SP)were observed,mainly involving two metabolic pathways,sphingolipid metabolism and fatty acid degradation,and could be one of the reasons for the neurobehavioral changes.Lipids such as ceramide(Cer),lysophosphatidylcholine(LPC),triglyceride(TG)fatty acid amides and their analogs may be potential lipotoxic biomarkers of AFB1-induced metabolic dysfunction.Based on the above studies,this thesis describes the disturbance of lipid metabolism caused by AFB1 and reveals the potential link between the abnormal neurobehavior and disorders of lipid metabolism.It is expected to provide reference for etiological investigation,early diagnosis and precise treatment of AFB1.