Effects Of Antidepressants Fluoxetine And Paroxetine On 5-HTergic Neurotransmission System In Zebrafish Brain Tissue

Depression is a common mental illness.With the intensification of social competition and the accelerated pace of life,the prevalence of depression is increasing year by year.The World Health Organization believes that the dangers of depression are second only to heart disease.The use of antidepressants is increasing due to the increasing number of people with depression.SSRI antidepressants are currently the most widely used class of antidepressants,and the purpose of treating depression is achieved by increasing the 5-HT concentration in the synaptic cleft.At present,the environmental pollution caused by drugs has gradually attracted the attention of scholars.Humans discarded and metabolized incompletely excluded drugs in vitro due to their inability to be well removed,resulting in widespread presence in surface water,ocean and other aquatic environments,seriously endangering the health of aquatic organisms.In this study,the representative anti-depressant drugs of fluoxetine and paroxetine were selected to explore the 5-HT_1AA receptor and 5-HT transporter?5-Hydroxytryptamine?,a key function of 5-HT-energy nerve conduction system in zebrafish brain tissue.Transporter,SERT)The effects of transcription and expression levels on the effects of SSRI-like antidepressants on the 5-HT neurotransmission system in zebrafish brain tissue.Adult zebrafish were continuously exposed to fluoxetine and paroxetine for 7 days and 14 days,respectively,and 5-HT_1A and SERT transcription and protein expression levels were measured after exposure.The results showed that fluoxetine and paroxetine caused an increase in the transcription levels of5-HT_1A and SERT genes,accompanied by a decrease in the expression level of SERT protein.The results of this study suggest that the SSRI antidepressants fluoxetine and paroxetine affect 5-HT_1A receptor and SERT levels in zebrafish brain tissue,affecting 5-HT synaptic structure and interfering with zebrafish brain tissue 5-HT It can normalize the physiological function of the nerve conduction system and harm the health of the zebrafish.This study provides a scientific basis for further study of the adverse effects of SSRI antidepressants on the central nervous system of zebrafish,and provides a reference for studying the effects of other drugs on the health damage of aquatic organisms.