MiR-495 Regulated The Malignant Phenotype Of Esophageal Squamous Cell Carcinoma Cells Via Suppressing The Expression Of RNF113A

OBJECTIVE:To investigate the effect of micro RNA-495(miR-495)on the malignant phenotype of esophageal cancer cells and its molecular mechanism.METHODS: MiR-495 mimetics,miR-496 inhibitors and their corresponding random sequence controls were transfected into esophageal cancer cell Eca109.QPCR was used to detect the expression of miR-495,CCK-8 was used to detect proliferation in each group,and flow cytometry was used to detect changes in cell cycle and apoptosis,transwell test was used to detect the cell migration and invasion ability of each group,and q PCR and Western blot test were used to detect the m RNA and protein expression of downstream target gene RNF113 A.RESULTS: Compared with the control group,the miR-495 mimetic transfection group showed a significant increase in miR-495 m RNA levels(P<0.05),while the miR-465 m RNA levels in the miR-495 inhibitor transfection group decreased significantly(P<0.05).There was no significant change in cell proliferation between the two groups(P>0.05).The number of cell migration and invasion in the miR-495 simulant transfection group was significantly reduced(P<0.01),and the rate of apoptosis was significantly increased(P<0.05).The cell cycle was blocked in G0/G1phase(P<0.001),with a statistically significant difference;The number of migration and invasion in the miR-495 inhibitor transfection group significantly increased(P<0.01),while the apoptosis rate significantly decreased(P<0.01).The proportion of S-phase cells in the miR-495 inhibitor transfection group increased(P<0.05),and the difference was statistically significant.Compared with the control group,the expression of RNF113 A protein was downregulated in the miR-495 mimetic transfection group(P<0.05),while the expression of RNF113 A was upregulated in the miR-495 inhibitor transfection group(P<0.05).CONCLUSION:miR-495 can regulate the expression of RNF113 A protein,inhibit its migration and invasion ability,regulate cell cycle distribution,and mediate the malignant phenotype of esophageal cancer cells.