The Study Of Correlation Of CDCA5 And Acute Leukemia

Background: Acute leukemia is a disease with highly heterogeneous molecular mechanisms and clinical features.CDCA5(Cell Division Associated 5)is a chromosomal adhesion regulatory protein that regulates the correct segregation of sister chromatids and gene repair,and is an important mechanism for the genetic stability of highly proliferating cells.Current studies have shown that CDCA5 is associated with a variety of tumors including lung,gastric,colorectal,liver,bladder,and breast cancers,and its expression is significantly elevated in tumor cells,and is closely related to tumor malignancy,disease progression,stage,metastatic risk,and patient survival time,making it a potential therapeutic target molecule.However,fewer studies have been conducted to correlate CDCA5 with acute leukemia.Therefore,we proposed to analyze and compare the expression level characteristics of CDCA5 in acute leukemia cells and the relationship with disease status and prognostic characteristics.Purpose:(1)To explore the expression characteristics of CDCA5 in acute leukemia cells;(2)To analyze the correlation between CDCA5 expression in acute leukemia cells and clinical features such as disease status and disease prognosis.Methods:1.The differences in CDCA5 expression in various tumor tissues and normal tissues were detected through the cancer genome atlas(TCGA),the Therapeutically Applicable Research to Generate Effective Treatments(TARGET)and UALCAN databases.The correlation between CDCA5 expression and clinical features of AML was analyzed in the UALCAN database.The correlation between CDCA5 expression and prognosis in acute myeloid leukemia was analyzed using survival analysis.The significance of CDCA5 in the development of acute leukemia was explored using the STRING database to construct a CDCA5-related reciprocal protein network with GO gene function enrichment analysis and KEGG pathway enrichment analysis.2.Paired detection of CDCA5 m RNA and protein expression levels in the bone marrow of acute leukemia patients attending the Department of Hematology of the First Hospital of Lanzhou University at initial diagnosis and after remission to validate the results of database analysis and explore the differences in CDCA5 expression in acute leukemia cells(unremitting bone marrow)and normal cells(post-remitting bone marrow)and the clinical prognostic value.Results:1.By exploring TCGA and GEPIA databases,it was found that CDCA5 expression was significantly upregulated in most tumor tissues compared with normal tumor-derived cells and tissues,but CDCA5 expression was significantly downregulated in acute myeloid leukemia bone marrow compared with normal bone marrow;further exploring the relationship between CDCA5 and clinicopathological features of acute myeloid leukemia using UALCAN database It was found that CDCA5 was significantly correlated with gender and possibly correlated with FAB typing;the relationship between CDCA5 expression and prognosis of AML patients was analyzed by TCGA,TARGET data and KM survival,and it was found that patients with high CDCA5 expression had shorter survival,but CDCA5 was not sufficient as an independent factor to judge patient prognosis.Analysis of the functional pathway of CDCA5 gene showed that CDCA5 is mainly involved in cell division and proliferation and immune processes.By GO functional enrichment analysis CDCA5 is mainly involved in organelle division,nuclear division,chromosome segregation and mitotic division,adenosine triphosphatase activation,microtubule protein binding,microtubule binding and other biological pathways,which are closely related to value-added cell division.In AML immune checkpoints TIGIT(P < 0.01)and HAVCR2(P < 0.05)differed statistically between groups with high and low CDCA5 expression.In terms of cellular infiltration,CDCA5 expression in AML was significantly negatively correlated with T cells(P < 0.05),NK cells(P < 0.05)and monocytes-macrophages(P < 0.01).2.CDCA5 m RNA expression was upregulated in acute leukemia patients after complete remission compared to initial diagnosis,(P = 0.035).c DCA5 protein expression was upregulated in acute leukemia patients after complete remission compared to initial diagnosis(P = 0.092).The present study showed a trend of upregulation of CDCA5 gene expression in remission samples compared to onset,consistent with the database analysis.Conclusion:1.CDCA5 expression is reduced in acute leukemia relative to normal bone marrow and correlates with age,gender,and FAB typing;CDCA5 levels may correlate with AML prognosis in children;CDCA5 levels correlate with immune checkpoint of TIGIT and HAVCR2 expression in AML and infiltration with T cell,NK cell,and monocyte-macrophage.2.CDCA5 gene expression tended to be upregulated in acute leukemia remission samples compared to the onset.