Targeted Inhibition Of CDCA5 Gene Suppresses The Proliferation Of Myeloid Leukemia Cells And Mechanisms

Objective: To investigate the effect of targeted inhibition of the CDCA5 gene on the proliferation of myeloid leukemia cells and its mechanism.Methods: The CDCA5 specific siRNA lentivirus vector was constructed,and the interference effect of the target gene was exogenously verified by Western blot with293 T tool cells.Myeloid leukemia cell lines,HL-60 and K562,were transfected with specific siRNA(shCDCA5)-containing lentivirus and negative control(sh-Ctrl),and the cellular morphology and transfection efficiency were observed under a fluorescence microscope.The level of CDCA5 mRNA was detected by PCR.Cellular proliferation was detected using the Cell Counting Kit-8(CCK-8),and apoptosis was detected using Annexin V-APC single staining via flow cytometry and evaluation of caspase 3/7 activity.Results: The expression of FLAG-tag of CDCA5 decreased significantly by shCDCA5 in 293 T cells proved an effective siRNA interference.After the transfection of lentivirus cells,the transfection efficiency was over 80%.The relative expression levels of CDCA5 gene in HL-60 and K562 reduced to 37.7 ± 3.7% and 19 ± 0.9%,respectively,confirmed that siRNA inhibited the transcription of CDCA5 gene.In the HL60 cells and K562 cells experimental groups,the rate of proliferation was inhibited and the level of apoptosis was increased.Conclusion: Targeted inhibition of CDCA5 can inhibit the growth of myeloid leukemia cells,which is related to the increase of apoptosis.This gene may be used as a potential molecular target in the treatment of myeloid leukemia.