Expression Of CD133、GD2 And CD10 In Invasive Micropapillary Carcinoma Of Breast

Objective: Based on Non-specific invasive ductal carcinoma(IDC-NOS)and adjacent tissues of breast cancer as control,we investigated the expression of CD133,GD2 and CD10 in invasive micropapiliary carcinoma(IMPC),and discussed whether the clinicopathological features and prognosis of IMPC are related to the three proteins.Methods: The clinical data of breast cancinoma underwent surgery from The Department of Pathology of Baoding First Central Hospital from January 2017~ January 2022 were collected,including 54 cases of IMPC,56 cases of IDC-NOS,and 20 cases of adjacent normal tissue.The expression rate of CD133,GD2 and CD10 proteins in each group was detected by using Immunohistochemistry(Envision two-step)assay,and the relationship between three proteins and the clinicalpathological parameters of two groups of cases was evaluated.The survival curve of each group was compared by Log rank test,and COX proportional risk model were used to evaluate the relevant prognostic factors of the two groups of cases.The Medical Ethics Committee had approved and supported study on the subject.Results: 1.Comparison of clinical pathological features between IMPC group and control group The maximum tumor diameter(P=0.007)and ER positive rate(P=0.040)of IMPC were significantly bigger than those of IDC-NOS.2.Expressions of CD133,GD2 and CD10 in each group The level of expression was higher of CD133 and GD2 in IMPC than that in IDC-NOS and para-tumor tissues(P<0.05);The expression of CD10 in IMPC and IDC-NOS had no significant difference(P>0.05),but was higher than that in paracancer tissues.3.Relationship between CD133,GD2 and CD10 and clinicopathologic features of each group In IMPC and IDC-NOS,the number of p TNM stages and lymph node metastases were positively correlated with CD133,and CD133 was negatively associated with PR.In both groups,histological grade,p TNM stages and number of lymph node metastasis were positively correlated with GD2,and GD2 was negatively associated with PR.In IMPC,The stromal expression of CD10 protein was positively correlated with tumor maximum diameter,HER2 and Ki67.In IDC-NOS group,CD10 was all related to number of lymph node metastasis,ER,HER2,Ki67 and p TNM staging.4.Correlation analysis of CD133,GD2 and CD10 proteins in prognosis of breast cancinoma There was no significant difference in either OS or DFS between IMPC and the control IDC-NOS(P>0.05).In IMPC group,Not only were CD133,GD2,PR,number of lymph node metastases closely related to disease-free survival(DFS)of the patients,but also affected the patients’ the overall survival(OS).In IDC-NOS group,CD133,CD10,p TNM stages and lymph node metastases all affected survival outcome of the patients.And CD133 was the independent prognostic factor for IMPC.Conclusions: 1.The expression rates of CD133 and GD2 proteins in IMPC were higher than those in IDC-NOS and adjacent breast cancer tissues.2.CD133,GD2,or CD10 were significantly associated with IMPC signaling and lymph node invasion.The three may synergistically regulate the infiltration and metastasis of tumor cells in IMPC.3.CD133 expression was associated with poorer survival outcome in patients with IMPC.CD133 could be a Potential biomarker for assessing and predicting the adverse biological behavior of IMPC.