Differential Proteins Expression In Plasma Of Asphyxiated Neonates With Hypoxic-ischemic Encephalopathy Based On Proteomics Technology

Objective: To identify and analyze the expression of plasma proteins in asphyxiated children with neonatal hypoxic-ischemic encephalopathy by 4D Label-free proteomics technology,so as to screen the differential proteins and enrichment related to the pathogenesis of neonatal hypoxic-ischemic encephalopathy,and these differential proteins were analyzed by bioinformatics technology,in order to explore the potential biomarkers of neonatal hypoxic-ischemic encephalopathy caused by different degrees of asphyxia,and provide a research basis for early diagnosis and intervention.Methods: According to the inclusion and exclusion criteria,plasma samples of 21 eligible newborns and healthy newborns were collected,including 7 cases in group A(control group),7 cases in group B(mild asphyxia group),and 7 cases in group C(severe asphyxia group).)7 cases were tested by 4D Label-free proteomics,and differential proteins were screened through bioinformatics analysis,and functional enrichment analysis was performed on the screened differential proteins through GO functional annotation and KEGG pathway annotation.Results: In this study,A total of 74 differential proteins were identified in group A and group B,among which 48 were up-regulated and 26 were down-regulated.GO analysis showed that the differential proteins were concentrated on the cytoskeletal anchorings and complement activation processes,extracellular space,and serine-type endopeptidyase activity.122 proteins were detected in group A and Group C,81 were up-regulated and 41 were down-regulated.GO enrichment showed that differential proteins were concentrated in glycogenogenesis,extracellular space,and threonine endopeptide activity.In group B and C,10 proteins were identified,with 6 up-regulated proteins and 4 down-regulated proteins.The differential proteins were concentrated in the plasma membrane by GO analysis.KEGG analysis showed that the most significant pathway of differential protein enrichment was metabolic pathway.Conclusion: In this study,4D Label-free proteomics analysis was used to screen differential proteins in the plasma of asphyxiated children with hypoxic-ischemic encephalopathy.We screened out differential proteins and found that the most significant pathways for differential protein enrichment were metabolic pathways.The results of this study can provide a molecular biological basis for the further exploration of early diagnosis and intervention of asphyxia with neonatal hypoxic-ischemic encephalopathy.