Study On Improvement Of Axillary Osmidrosis By Lactobacillus And Its Effect On Axillary Skin Microecology

Objective: A casual survey was conducted on the body odor of urban residents around the school to preliminarily understand the prevalence of axillary osmidrosis among residents in this area.To explore the effect of Lactobacillus on the skin microecology of patients with axillary osmidrosis and find a new way to treat axillary osmidrosis.Methods:Questionnaire survey: an electronic questionnaire survey was conducted among the urban residents around the school.The questionnaire was designed with 6 questions,including: gender,age range,whether you can smell the pungent underarm odor after exercise,the degree of odor after exercise,whether you think this situation is underarm odor,and whether parents have similar situations.2-4 options are attached to each question.Collecting and analyzing the collected data after the investigation.Experiment: We recruited 10 volunteers with bromhidrosis.Volunteers are required to be healthy without acute or chronic diseases and no recent treatment of treat axillary osmidrosis within 4 weeks.Before the experiment,the volunteers were graded for bilateral armpit odor and analyzed by 16 S r DNA sequencing.10 ml normal saline mixed with 2g Lactobacillus was used as experimental spray,and pure normal saline was used as control spray.All volunteers were given Lactobacillus mixed saline spray to their left armpit(experimental group)and saline spray to their right armpit(control group)after daily bathing for 28 consecutive days.After the experiment,16 S r DNA analysis was performed on the bacteria in both armpits of volunteers to compare the changes in bacterial abundance.After the experiment,the volunteers were rated for bilateral armpit odor.SPSS 25.0 tool was used to analyze the data,and paired T test was used to analyze the main bacterial abundance before and after the experiment.Shannon index and Simpson index were used to examine the safety of treatment.Results:Questionnaire results: the proportion of axillary pungent smell after exercise is 38.75% among people aged 18-35,35.29% among people aged 36-50 and 45.71% among people over 50.Experiment result: After 28 days of continuous use of Lactobacillus,the sample score of 10 cases in the experimental group decreased in 9cases,and the sample score of 1 case remained stable.At the same time,in the control group,only 2 samples decreased their scores.The experimental group score was significantly lower than the control group(P = 0.013).In the microbial analysis,the first seven bacterial genera were selected,and the two dominant bacterial genera were Corynebacterium and Staphylococcus.The abundance of Corynebacterium decreased by(18.6% ± 5.6%)in the experimental group;The abundance of Corynebacterium increased by(6.2% ± 4.5%)in the control group.The abundance of Corynebacterium was also significantly decreased(P <0.01).There was no significant difference in the abundance of Staphylococcus aureus between the two groups.According to the T-test results,no significant variation in the microbe diversity before and after the treatment can be found in Shannon index and Simpson index,which indicated the diversity of axillary surface microecology was not damaged.Conclusion: The questionnaire to schools and surrounding residents shows that more than 30% of the respondents have obvious body odor after exercise;Using lactobacillus for a period of time can reduce the abundance of Corynebacterium and improve axillary osmidrosis.This method does not cause significant changes in bacterial diversity.Lactobacillus preparation may become a new way to treat axillary osmidrosis.