| Allium mongolicum Regel is a kind of onion plant with high nutritional and medicinal value.In this thesis,firstly,the Allium mongolicum Regel was treated with water extraction lyophilization and drying to prepare water extraction Allium mongolicum Regel lyophilized powder(WAF)and Allium mongolicum Regel dried powder(DA);the content of main active ingredients in WAF and DA was determined;the hypolipidemic and anti-inflammatory effects of WAF and DA were investigated by establishing nematode and mouse hyperlipidemic models;finally,the effects of WAF and DA on intestinal flora were determined by intestinal flora analysis,as follows : The effects of WAF and DA on intestinal flora were determined through intestinal flora analysis:1、Determination of the component content and antioxidant activity of WAF and DAIn this chapter,the freeze-dried and dried Allium mongolicum Regel was treated with water extraction,and the polysaccharide,flavonoid and chlorophyll contents in the freeze-dried water extracted Allium mongolicum Regel powder and dried Allium mongolicum Regel powder were determined by spectrophotometry;the polysaccharide,flavonoid and chlorophyll contents in the digested water extracted Allium mongolicum Regel freeze-dried powder and dried Allium mongolicum Regel powder were determined by simulating artificial gastrointestinal fluid,in which the polysaccharide was digested by 14.99% and 8.46%,the flavonoid was digested by10.95% and The digested polysaccharides,flavonoids and chlorophylls were 14.99%and 8.46%,flavonoids were 10.95% and 4.46%,chlorophyll a was 12.38% and13.95%,chlorophyll b was 36.54% and 33.90%,and carotenoids were 31.12% and44.98%,indicating that polysaccharides,flavonoids and chlorophylls were not completely decomposed and digested in the stomach and small intestine;through the determination of the scavenging ability of DPPH free radicals by the Allium mongolicum Regel extract,it showed that the Allium mongolicum Regel extract has antioxidant activity and can improve the oxidative stress capacity,which can play a certain degree of prevention on hyperlipidemia.In this chapter,the sand onion was freeze-dried and dried in water,and the polysaccharides 285.6mg/g,flavonoids12.88mg/g,chlorophyll a 29.48mg/g,chlorophyll b 17.73mg/g,carotenoids 9.19mg/g in the freeze-dried powder of sand onion(WAF)were determined by spectrophotometry;the polysaccharides 152.6mg/g,flavonoids 10.53mg/g,chlorophyll a 27.38mg/g,chlorophyll b 14.75mg/g,carotenoids 7.67mg/g in the dried powder of sand onion(DA).The changes in polysaccharide,flavonoid and chlorophyll contents were measured by simulating artificial gastrointestinal digestion,in which WAF and DA polysaccharide were digested by 14.99% and 8.46%,respectively,and flavonoid was digested by 10.95% and 4.46%,respectively.The experimental results showed that polysaccharides,flavonoids and chlorophylls were not fully digested in the stomach and small intestine,and the proportion of each component digested in WAF was higher than that in DA.The results showed that polysaccharides,flavonoids and chlorophyll were not fully digested in the stomach and small intestine,and the proportion of WAF components digested was higher than that of DA.Studies in the literature have shown that antioxidant activity has a positive correlation with lipid lowering.Therefore,WAF and DA may have hypolipidemic functions.2、Study on the effects of WAF and DA on N2 lipid reduction,longevity,locomotion and reproduction in Cryptobacterium hidradenumIn this chapter,a high-fat model was established using Cryptobacterium hidradenum N2,and the lipid-lowering activities of WAF and DA were measured by oil-red O staining experiment,and the experimental results showed that the WAF and DA groups reduced the fat content of high-fat nematodes by 48% and 43%,respectively,compared with the high-fat model group;by the longevity experiment,the WAF and DA groups extended the average lifespan of high-fat nematodes by22.2% and 16.7%,respectively,compared with the high-fat model group By motility experiment,both WAF and DA groups increased the average number of oscillations of high-fat nematodes by 18.2% compared with the high-fat model group;by reproduction experiment,WAF and DA groups increased the total egg production of high-fat nematodes by 18.4% and 13.5% compared with the high-fat model group,respectively.In conclusion,WAF and DA have certain function of lipid reduction and affect the longevity,motility and reproduction by improving the high lipid content of nematodes,and the function of WAF is better than DA.3、Study on the hypolipidemic and anti-inflammatory effects of WAF and DA on high-fat miceIn this chapter,a high-fat diet-induced hyperlipidemia model was established using KM mice.By comparing the food intake,body weight and organ weights of mice in the administered and blank groups,it was shown that WAF and DA could effectively reduce the food intake,body weight and the increase in heart,kidney,liver and abdominal fat weights in high-fat mice;by detecting the serum levels of TC,TG,LDL-C and HDL-C in mice,it was shown that WAF and DA could decrease TC,TG and LDL-C contents and increase HDL-C contents in high-fat mice;by HE staining,it was shown that WAF and DA could improve abnormal lipid accumulation in mouse liver;by detecting TNF-α and IL-1β contents in mouse liver tissues,it was shown that WAF and DA could improve liver inflammation caused by hyperlipidemia.4、Study on the effect of WAF and DA on the regulation of intestinal flora in high-fat miceIn this chapter,through gut microbial species composition analysis,it was found that both high-fat diet and WAF and DA affect the number of microorganisms in the gut at each taxonomic level;through Alpha diversity analysis,it was found that WAF and DA can restore the decrease in gut microbiota richness and diversity induced by high-fat diet;through Beta diversity analysis,it was found that there is some intestinal microflora between different groups By Beta diversity analysis,we found that there was some variability in the gut microbiota among different groups;by species differences and marker species analysis,we found that WAF and DA could reduce the increase in the ratio of thick-walled phylum aphyllobacteria in the gut flora caused by high-fat diet.At the genus level,the relative abundance of Coprococcus,Akkemansia and Oscillospira were higher in the WAF group,while the relative abundance of Bifidobacterium was higher in the DA group.Therefore,it is hypothesized that WAF and DA lowered lipids by affecting the abundance of SCFAs-producing bacteria in the intestinal microflora of mice,and that WAF increased the number of beneficial bacteria more and had a better improvement of the intestinal environment.In this thesis,we determined that WAF and DA have hypolipidemic,anti-inflammatory and gut microbial improvement effects through the above studies,and laid the theoretical foundation for the development of WAF and DA as foods and drugs to improve hyperlipidemia. |
