| Background:Acute myocardial infarction(AMI)is a common cardiovascular disease among middle-aged and elderly people,characterized by high mortality and disability rates.The current treatment modalities is mainly reperfusion myocardial therapy,which can reduce the area of the myocardial infarction and enhance the clinical prognosis of patients.However,studies have found that cardiac function deterioration and structural damage to myocardial tissue occur during reperfusion,an injury we call myocardial ischemia-reperfusion injury(MIRI).Since the occurrence of MIRI is rarely predicted in advance in the treatment of AMI patients,the prevention of reperfusion injury to myocardial cells in AMI patients to improve their condition and the prognosis is key issues that need to be tackled urgently in the contemporary medical environment.Initial progress has been made in the study of MIRI for a long time,and its development includes the involvement of processes such as massive production of reactive oxygen species,calcium overload,inflammatory response,disturbance of energy metabolism,and programmed cell death.Inflammatory response is considered to be one of the important mechanisms of MIRI,and excessive inflammatory response is considered to be the main driving force in MIRI.Inhibition of inflammatory response can limit myocardial infarct size and reduce myocardial ischemia-reperfusion injury thereby improving cardiac function.How to take effective measures to inhibit the excessive occurrence of inflammatory response during MIRI is a growing concern for researchers both at home and abroad.The traditional Chinese herb Curcuma longa is widely used in the clinical treatment of various diseases for its multiple pharmacological activities.Curdione,a sesquiterpenoid isolated from the volatile oil of Curcuma longa,has multiple pharmacological effects such as antioxidant,anti-fibrotic,anti-platelet aggregation and anti-tumor,which is a classical herb for promoting blood circulation and resolving blood stasis in ancient China and also plays an important role in modern herbal treatment.It has been found that curdione have cardioprotective effects in animal models of cardiotoxicity;however,whether curdione have protective effects against MIRI and their possible related mechanisms are still unclear.Objective:To investigate the effect of curdione on myocardial ischemia-reperfusion injury in the rats and to reveal its possible molecular mechanism.Methods:Animal experiments:Forty 8-week-old adult male SD rats were randomly divided into 4 groups(n=10 for in each group): control group,modeling group,low-dose curdione group(20 mg/kg/day)and high-dose curdione group(60 mg/kg/day).The control group and the model group were given 0.4% dimethyl sulfoxide by gavage continuously for 1 week,and the low-dose curdione group and the high-dose curdione group were given20 mg/kg/day and 60 mg/kg/day of curdione by gavage for 1 week,respectively.One week later,the rat MIRI model was constructed by tying the left anterior descending coronary artery in all groups,and only threading without ligation was performed in the control group.After the end of modeling,the changes of left ventricular end-systolic volume and left ventricular end-diastolic volume in each group of rats were observed by ultrasound.The rats were executed,the area of myocardial infarct was observed by 2,3,5-triphenyltetrazolium chloride(TTC)staining,myocardial apoptosis was observed by Td Tmediated d UTP nick-end labeling(TUNEL)staining,and enzyme-linked immunosorbent assay(ELISA)assay for the detection of inflammatory factors such as tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)in myocardial tissues.Results:(1)In comparison with the control group,LVESV and LVEDV were significantly elevated in the modeling group(P < 0.05),an enlargement of myocardial infarct area(P < 0.05),an increase in the proportion of myocardial apoptosis(P < 0.05),and an increase in TNF-α,IL-1β and IL-6 contents(P < 0.05);(2)When comparing with the modeling group,the low-dose curdione group and the high-dose curdione group both showed a decrease in LVESV and LVEDV(P < 0.05),a narrowing of the infarct area(P <0.05),a reduction in the proportion of apoptotic cardiomyocytes(P < 0.05),and a decrease in TNF-α,IL-1β and IL-6 contents(P < 0.05);and the efficacy of the high-dose curdione group was superior to that of the low-dose curdione group(P < 0.05).Conclusions:Curdione protects against myocardial ischemia-reperfusion injury(MIRI)in rats and can exert myocardial protective effects by inhibiting inflammatory reaction in MIRI. |
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