Effect Of LncRNA SLCO4A1-AS1 On Proliferation And Migration Of Thyroid Papillary Carcinoma Through WNT/β-catenin Pathway

Objectives To explore the effect of LncRNA SLCO4A1-AS1 on thyroid papillary carcinoma cells and its possible signaling pathway.Methods 1 The inhibitory group model was constructed by the instantaneous transfection technique of interfering RNA,and the meaningless sequence was transfected as the inhibitory control group.According to different inhibitory targets,the inhibitory group was divided into inhibitory group(1),inhibitory group(2)and inhibitory group(3).SLCO4A1-AS1 was transfected with SLCO4A1-AS1 to construct the overexpression group model,and the empty plasmid was transfected as the overexpression control group.Cells left untreated served as a blank group.2 The expression levels of SLCO4A1-AS1 in human thyroid papillary carcinoma cell TPC-1 in blank group,inhibited group,inhibited control group,overexpression group and overexpression control group were detected by q RT-PCR to verify whether the cell model was successfully constructed.3 In vitro cell function test:proliferation ability of human thyroid papillary carcinoma cells was detected by CCK8,migration ability of human thyroid papillary carcinoma cells was detected by scratch assay,and invasion ability of human thyroid papillary carcinoma cells was detected by Transwell invasion assay.4 Western blot was used to detect the expression of β-catenin and AXIN2 protein in Wnt/β-catenin pathway.Results 1 The expression level of SLCO4A1-AS1 in the inhibitory group was compared with that in the blank group and the inhibitory control group,and the difference was statistically significant(P<0.05).The expression level of SLCO4A1-AS1 in overexpression group was significantly different from that in blank group and blank control group(P<0.05).The expression levels of SLCO4A1-AS1 in blank group,inhibited group and overexpressed group were also significantly different(P<0.05).Among the three inhibitory groups with different targets,inhibitory group 2 had the best inhibitory effect,inhibitory group(2)was retained,and inhibitory group(2)was used in subsequent experiments.2.The increment ability,migration ability and invasion ability of SLCO4A1-AS1 cells were significantly reduced after knockdown,and the difference was statistically significant(P<0.05);Overexpression of SLCO4A1-AS1 significantly enhanced the proliferation ability,migration ability and invasion ability of SLCO4A1-AS1 cells,with statistical significance(P<0.05).3.Western blot showed that the expression levels of β-catenin protein and AXIN2 protein were decreased after SLCO4A1-AS1 knockdown,and the differences were statisticallysignificant(P<0.05).Overexpression of SLCO4A1-AS1,β-catenin protein and AXIN2 protein was significantly increased,and the difference was statistically significant(P<0.05).Conclusions 1 The stable cell model of TPC-1 cells with suppressed and overexpressed human papillary thyroid carcinoma was successfully established by real-time fluorescence quantitative PCR,and the inhibitory group(2)with the best inhibitory effect was selected as the inhibitory group for subsequent experiments.2 SLCO4A1-AS1 significantly promotes cell proliferation,migration and invasion in human thyroid papillary cell carcinoma.3.LncRNA SLCO4A1-AS1 may mediate the occurrence and development of thyroid papillary cell carcinoma through the Wnt/β-catenin pathway.Figure8;Table12;Reference 142...