Effect Of Anwei Decoction On The Expression Of Ulk1-related Autophagy Protein In Rats With Chronic Atrophic Gastritis

Objective: To investigate whether the mechanism of Anwei Decoction in the treatment of chronic atrophic gastritis(CAG)rats is related to autophagy.Methods: 72 SD rats were randomly divided into 6 groups: normal control group,model control group,experimental observation AH group(high-dose Anwei decoction group),experimental observation AM group(medium-dose Anwei decoction group),experimental observation AL group(low-dose Anwei decoction group),negative control group(normal saline group).The model of chronic atrophic gastritis was established by free drinking with ammonia and sodium deoxycholate solution and feeding with hunger disorder.Then give different concentration of Anwei decoction intragastric treatment after 4 weeks.The pathological changes of gastric tissues and the expression of autophagyrelated genes and proteins in rats were observed.Results: The pathological results showed that compared with the negative control group,the gastric gland atrophy in each treatment group of Anwei Decoction decreased to different degrees,and the infiltration of lymphocytes,plasma cells and acid cells in the lamina propria decreased.In real-time PCR detection,the expressions of ULK1,Atg13,Beclin-1,LC3 m RNA in each treatment group of Anwei Decoction were significantly up-regulated(P<0.05).Western blot showed that ULK1,Atg13,Beclin-1 and LC3 proteins were also up-regulated in anwei Decoction treatment groups(P< 0.05).Finally,in the transmission electron microscope test results,no significant autophagy or minimal autophagy was found in the normal control group,model control group and negative control group,while different amounts of autophagy lysosomes were found in each anwei decoction treatment group.Conclusion Anwei Decoction can improve the relative expression of ULK1,Atg13,Beclin-1 and LC3 proteins in tissues while improving chronic atrophic gastritis,suggesting that the therapeutic mechanism of anwei decoction is related to autophagy.Conclusions: 1.Anwei Decoction can treat chronic atrophic gastritis in rats induced by sodium deoxycholate and ammonia and repair the damage of gastric mucosa.2.Anwei decoction can up-regulate the relative expression of ULK1,Atg13,Beclin-1 and LC3 autophagy factors in the gastric tissue of rats with chronic atrophic gastritis.3.When Anwei Decoction interferes with the chronic atrophic gastritis induced by sodium deoxycholate and ammonia,the number of autophagy lysosomes in the gastric tissue cells can be increased.