| Objective:To establish a model of tibial transverse transport in type 2 diabetes rats.Materials and Methods:36 SD rats were induced into type 2 diabetes models by feeding them with high glucose and high fat combined with low-dose streptozotocin,then,the successfully modeled rats were randomly divided into a control group,a fixed group,and a transverse transfer group,with 12 rats in each group.All rats have established acute wound model on the dorsum of the foot,and the rats in the fixator group and the tibial transverse transport group were operated on by using the selfdeveloped and designed rat special tibial transverse transport device.After surgery,the tibial transverse transport group was transported.On day 0,3,6,9,12 and 15,the photos of the foot dorsum wound were taken respectively,and the wound non-healing rate at each time point was calculated.On day 9 and 15 day after operation,6 rats in each group were randomly selected for enzyme-linked immunosorbent assay to determine the expression of endothelial vascular growth factor-A(VEGF-A),basic fibroblast growth factor(bFGF),platelet-derived growth factor(PDGF),interleukin-10(IL-10),stromal-cell derived factor-1 alpha(SDF-1α)and granulocyte colonystimulating factor(G-CSF)in peripheral blood serum.Results:All rats were successfully induced into type 2 diabetes model.And the rat special tibial transverse transport device can stably and continuously stretch bone blocks.On day 9,12 and 15,the wound non-healing rate in the tibial transverse transport group was significantly reduced than other groups(P<0.05).On day 9 and 15.the expression of VEGF-A,bFGF,PDGF,IL-10,SDF-1α and G-CSF in peripheral blood serum was significantly increased than other groups(P<0.05).Conclusion:The model of tibia transverse bone transfer in type 2 diabetes rats was successfully established by using a self-designed device specially designed for rats,which is suitable for popularization and application in the mechanism research of tibial transverse transport technique. |
