Explore The Mechanisms Of Tibial Transverse Transport In The Treatment Of Diabetic Foot Ulcer Based On TGF-β1/Smad3 Signaling Pathway

Objective: To observe the effect of tibial transverse transport in the treatment of diabetic foot ulcer.Explore the mechanisms of tibial transverse transport in the treatment of diabetic foot ulcer based on TGF-β1/Smad3 signaling pathway,and provide theoretical support for tibial transverse transport technology.Methods:(1)24 rabbits were randomly divided into 4 groups,control group: ordinary acute wound,model group: diabetic foot ulcer wound,TGF-β1 group: diabetic foot ulcer wound treated with injection of TGF-β1,tibial transverse transport group: diabetic foot ulcer wound treated with tibial transverse transport technology.(2)28 days after treatment intervention,the repaired wound tissues of each group were collected and stored in a-20°C refrigerator.(3)Observe the healing conditions of each group on the 7th,14 th,and 28 th day after treatment,and calculate the wound healing rate.(4)Take the wounds of each group for HE staining.(5)Western Blot to detect the expression levels of TGF-β1 and Smad3 proteins.Results:(1)The wounds of the rabbits in the control group were basically healed;the wounds of the rabbits in the model group did not improve significantly,and some wounds were infected and exuded;the wounds of the rabbits in the TGF-β1 group were significantly reduced and healed well;the wounds of the rabbits in the tibial transverse transport group were basically healed,with some hair growing.Compared with the control group,the wound healing rate in the model group was significantly decreased(P < 0.05);compared with the model group,the wound healing rate in the TGF-β1 group and the tibial transverse transport group was significantly increased(P < 0.05),and there was no statistical difference between the two groups(P > 0.05).(2)In the control group,the number of new collagen fibers increased,fibroblasts proliferated in large numbers,arranged in clusters,and a few new capillaries appeared.In the model group,the number of inflammatory cells increased significantly,the arrangement of fibroblasts was disordered,and no obvious new capillaries were found.In the TGF-β1 group,a large number of fibroblasts were densely distributed around the capillaries,and a few new capillaries appeared.In the tibial transverse transport group,there were more collagen fibers,hypertrophic fibroblasts,dense distribution,and more new capillaries.(3)Comparison of TGF-β1 and Smad3 protein expressions in each group.The results of Western-blot detection showed that compared with the control group,the expression levels of TGF-β1 and Smad3 proteins in the model group were significantly decreased,and the difference was statistically significant(P < 0.05);Compared with the model group,the expression levels of TGF-β1 and Smad3 in the TGF-β1 group and the tibial transverse transport group were significantly increased,and the difference was statistically significant(P < 0.05),and the difference between the two groups was statistically significant(P <0.05),the tibial transverse transport group expression level was higher.Conclusion:(1)TTT technology has a significant effect on the treatment of DFU wound,which can speed up the wound healing speed,improve the quality of wound healing,and be close to the situ regeneration.(2)DFU wound is hard to heal associated with the low expression of TGF-β1 protein.(3)TTT technology affects TGF-β1/Smad3 signaling pathway,regulates the expression level of related proteins,affects the proliferation and crawling of epidermal cells,regulates the process of re-epithelialization,and promotes wound healing.(4)The less scar forming in DFU wound tissues repaired by the treatment of TTT technology whether due to the regulation of the TGF-β1/Smad3 signaling pathway cannot be determined.