| Objective:With the development and application of genetics,scholars at home and abroad have used different genetic testing methods to verify the association between Idiopathic Membranous Nephropathy(IMN)and M-type phospholipase A2 receptor(PLA2R)gene polymorphisms.The close association between IMN and PLA2R gene polymorphisms.However,the association varies among different regions and ethnic groups.In this study,eight single nucleotide polymorphism(SNP)loci on PLA2R were selected to investigate the association between IMN and PLA2R gene polymorphisms in Heilongjiang.Methods:In this study,35 patients with IMN diagnosed by renal puncture biopsy in Heilongjiang and 25 healthy controls were included.Early morning fasting venous blood was collected from both groups,DNA extraction and PCR amplification were performed,and PCR resequencing was applied to eight SNP loci of PLA2R(rs16844715,rs2715918,rs2715928,rs35771982 rs3749119,rs3828323,rs4665143,rs6757188)were genotyped and sequenced.The obtained genotypes and alleles were subjected to Hardy-Weinberg equilibrium law test;X~2test was used to analyse the difference in genotype and allele frequencies of each PLA2R locus between the IMN group and healthy controls;logistic regression was used to analyse the effect of each locus on IMN susceptibility under three genetic models:additive,dominant and recessive;the effect of IMN susceptibility genotype on The effect of IMN susceptibility genotype on different clinical indicators was analyzed.Application of multi-factor logistic regression to analyse the factors influencing IMN.Results:1.In this study,the incidence ratio of male to female was 4:1.The patients in the IMN group were divided into three groups according to the age classification criteria,including 3 male and 0 female cases in the young group,a total of 3 cases;18 male and 4female cases in the middle-aged group,a total of 22 cases;and 7 male and 3 female cases in the elderly group,a total of 10 cases.In other words,the composition of IMN was higher in males than females in all age groups,and the incidence of IMN was mainly in the age group of 45-59 years old,accounting for 62.86%of the total number of IMN patients.2.A X~2test between the IMN and healthy control groups showed a statistically significant difference in gender between the groups(x~2=21.212,p<0.05).Age,systolic blood pressure,diastolic blood pressure,total cholesterol,triglycerides,urea nitrogen,creatinine and uric acid levels were all higher in the IMN group than in the healthy control group,and albumin levels were lower than in the healthy control group,with statistically significant differences(p<0.05).3.The genotypes of all loci in IMN group and healthy group were consistent with Hardy Weinberg equilibrium law(P>0.05),and the frequency of GG genotype and G allele of rs35771982 locus in IMN group was higher than that in control group(X~2=7.229,P=0.016;X~2=9.521,P=0.002),CC genotype and C allele frequency of RS3749119 locus were higher than those of control group(X~2=8.350,P=0.015;X~2=11.810,P=0.001);There was no significant difference in genotype and allele frequency of rs16844715,rs2715918,rs2715928,rs3828323,rs4665143 and rs6757188 between IMN group and control group(P>0.05).4.rs35771982 in the additive model(G VS C),the risk of G allele was 5.056 times higher than that of C allele(OR=5.056,95%CI=1.684-15.177),In dominant models(GG VS CG+CC),the risk of GG genotype was 4 times higher than that of CG+CC genotype(OR=4.000,95%CI=1.158-13.817);rs3749119 in the additive model(C VS T)had a4.75-fold higher risk of C allele than T allele(OR=4.750,95%CI=1.871-12.061),In the dominant model(CC VS CT+TT),the risk of CC genotype was 4.333 times higher than that of CT+TT genotype(OR=4.333,95%CI=1.385-13.561),In recessive model(CC+CT VS TT),the risk of CC+CT genotype was 10.737 times higher than TT genotype(OR=10.737,95%CI=1.201-95.953);In IMN group,uric acid was statistically significant between GG genotype rs35771982 and CG+CC genotype group(P<0.05),and serum albumin was statistically significant between CC genotype rs3749119 and CT+TT genotype group(P<0.05).5.A multifactorial logistic regression analysis showed that gender,age and triglycerides influenced the occurrence of IMN(p<0.05).Conclusion:1.The polymorphisms of PLA2R SNP rs35771982 and rs3749119 were associated with IMN susceptibility,and the risk genotypes were GG genotype and CC genotype,and increased uric acid was associated with GG genotype,and hypoproteinemia was associated with CC genotype.2.Gender,age and triglycerides can affect the occurrence of IMN.The 35 IMN patients were mainly male,and the onset peak was 45-59 years old. |
