Association Of PLA2R1 Single Nucleotide Polymorphism With Idiopathic Membranous Nephropathy

Objective To investigate the susceptibility of M-type phospholipase A2 receptor1(PLA2R1)gene rs3749117 and rs4664308 with idiopathic membranous nephropathy(IMN).Methods Eighty-eight patients with IMN diagnosed by renal biopsy were selected as the case group,and 122 healthy controls in the physical examination center of the Autonomous Region People's Hospital were collected,to extract the object of study in the morning fasting venous blood,DNA extraction.The competitive allele-specific polymerase chain reaction(KASP)method was used to detect the rs3749117 and rs4664308 genotypes in the case group and the control group.STATA15.0 software was used to analyze whether the case group and the control group meet the test of hardy-weinberg law,and the non-conforming sites were discarded;SPSS21.0 was used to analyze the relationship between the rs3749117 and rs4664308 polymorphisms of PLA2R1 gene and the susceptibility to IMN,and the clinical data of kidney biopsy in IMN patients were collected to analyze the correlation between the polymorphisms of two sites of PLA2R1 gene and the clinical manifestations;Grouping was conducted according to age,and the susceptibility of rs3749117 and rs4664308 to IMN at different ages was analyzed by stratified x2 test.Results The rs4664308 did not meet the Hardy-Weinberg equilibrium;The genotype and allele frequencies of rs3749117 were statistically significant between the IMN group and the HC group(x2=23.415,P<0.001,x2=25.792,P<0.001);There was no significant difference in the age,gender,systolic blood pressure,diastolic blood pressure,urea nitrogen,creatinine,uric acid,triglyceride,total cholesterol,low density lipoprotein,albumin,urine protein and anti-PLA2 R antibody positive rate between rs3749117 TT genotype group and TC+CC genotype group(P>0.05);Divided into three groups according to age(youth group,middle-aged group and elderly group).The stratified chi-square test showed that there were differences in the distribution of susceptible genotype(TT genotype)and non-susceptible genotype(TC+CC genotype)between IMN and healthy controls in the youth and middle-aged groups(P<0.05),there was no difference in the distribution of rs3749117 susceptibility genotype(TT genotype)and non-susceptible genotype(TC+CC genotype)between the IMN and the healthy control in the elderly group(P>0.05);The PLA2R1 gene rs3749117 in the18-59 age group in the dominant model(TT VS CT + CC),recessive model(TT + CT VS CC),additive model(TT VS CC)and allele(T VS C)can increase the risk of IMN(OR = 5.509,95% CI = 2.816-10.778,P <0.001;OR = 3.626,95% CI = 1.009-13.036,P<0.001;OR = 7.083,95% CI = 1.909-26.277,P = 0.001;OR = 3.729,95% CI =2.174-6.396,P <0.001),the additive model(CT VS CC)cannot increase the risk of IMN(x2 = 0.014,P = 0.905);Multivariate logistic regression analysis showed that TT genotype,triacylglycerol,and total cholesterol at rs3749117 could affect the occurrence of IMN(P <0.05).Conclusion The PLA2R1 rs3749117 may be associated with susceptibility to IMN in young and middle-aged populations,and PLA2R1 rs3749117 may be unrelated to the occurrence of elderly patients with IMN;The risk of IMN may be higher in middle-aged people who carry the TT genotype than in young people.