| Objective:To compare the levels of mi RNA-206,Th17 cells and IL-17 in patients with asthma and the normal population.Meanwhile,the correlation between mi RNA-206 and Th17 cells,mi RNA-206 and IL-17 were analyzed.Finally,to explore the role and mechanism of mi RNA-206 in the pathogenesis of asthma,and provide new ideas for the treatment of asthma.Methods:The study selected 30 patients diagnosed with asthma as the experimental group,and20 healthy people as the control group.Fasting blood samples were collected on the next day.Real time quantitative PCR(q PCR)was used to detect the levels of mi RNA-206 in peripheral blood mononuclear cell of patients and healthy people.The number of Th17 cells in peripheral blood of the two groups was determined by Flow cytometry.The level of IL-17 was measured in patients with asthma and healthy controls by Cytometric Beads Array(CBA).Results:1.The expression level of mi RNA-206 was 0.10±0.09 in peripheral blood mononuclear cell of adult patients with acute asthma and 0.25±0.15 of healthy controls.The level of mi RNA-206 in asthmatic patients was lower than that in control group(P<0.05).2.The number of Th17 cells was 15.87±4.12/u L in peripheral blood of adult patients with acute asthma and 9.14±5.30/u L of healthy controls.The number of Th17 in asthmatic patients was higher than that in control group(P<0.05).3.The expression level of IL-17 was 15.26±4.01pg/m L in serum of adult patients with acute asthma and 6.54±2.32pg/m L of healthy controls.The level of IL-17 in asthmatic patients was higher than that in control group(P<0.05).4.The expression of mi RNA-206 in the experimental group had significant correlation with Th17 and IL-17.(Th17,r=-0.618,P<0.05、IL-17,r=-0.652,P<0.05).Conclusion:1.The decreased expression of mi RNA-206 in asthmatic patients is one of the pathogenesis of asthma.2.Th17 cells are elevated in asthmatic patients and secrete IL-17,leading to the asthma.3.The expression of mi RNA-206 was negatively correlated with IL-17 and Th17,suggesting that mi RNA-206 may be involved in the pathogenesis of asthma by regulating the differentiation and function of Th17 cells. |
