| Objective:The content of neutrophils and exosomes in peripheral blood of patients with preeclampsia was studied and the placental origin of exosomes in peripheral blood was detected.Methods:1.In this study,60 pregnant women who underwent regular prenatal examination in the Second Hospital of Shanxi Medical University from February 20 to July 20 were selected as the subjects.There were 30 cases in normal pregnant group and 30 cases in preeclampsia group;2.Neutrophils of the two groups were extracted and counted after primary culture;3.Two groups of anticoagulant peripheral blood were centrifuged to extract exosomes;4.The exosomes were identified by transmission electron microscope,and the concentration of exosomes in peripheral blood of the two groups was detected by detector;5.Western blot was used to detect exosome specific protein and placenta derived specific protein in peripheral blood of the two groups;6.Use spss22 0.The mean ± standard deviation was used to represent the experimental results.The comparison between the two samples was performed by independent sample t-test.Pearson correlation analysis was performed between neutrophils and exosomes in the two groups;7.The research scheme designed in this study has been supported and approved by the relevant departments and the Ethics Committee of the Second Hospital of Shanxi Medical University,and all subjects signed informed consent.Results:1.The neutrophils in peripheral blood content of experimental group(preeclampsia group)was higher than that in control group(normal pregnant women group).2.The content of exosomes in experimental group(preeclampsia group)was higher than that incontrol group(normal pregnant women group).3.The morphology of exosomes was observed by transmission electron microscope in preeclampsia group and normal pregnant women group.4.Western blot showed that both preeclampsia group and normal pregnant women group expressed specific protein CD63 and specific placental alkaline phosphatase.5.Pearson correlation analysis showed that there was a positive correlation between the content of neutrophils in peripheral blood and the concentration of exosomes(r =0.583,P <0.01).Conclusion:The content of exosome in peripheral blood was significantly positively correlated with the number of neutrophils,suggesting that placental derived exosomes may be involved in the activation of neutrophils.This results in vascular endothelial damage,which is related to the pathological mechanism of preeclampsia. |
