Discussion On The Correlation Between Placental Microbiota Imbalance And NLR With Preeclampsia

Background and Purpose:Preeclampsia(PE)is a pregnancy-specific hypertensive disease and a serious complication of pregnancy with multiple organ damage.PE has a high incidence and often leads to serious pregnancy outcomes.The current measures are timely termination of pregnancy and antihypertensive treatment,but there are no clear screening and effective treatment measures.It is believed that the cause of PE is related to oxidative stress at the maternal fetal interface and inflammatory stimulation in maternal circulation.As researchers studied the placenta’s microbes,they discovered a unique microbiome.The disturbance of bacterial flora can promote endothelial cell damage,and through its own or metabolites cause placental local inflammation and systemic inflammatory response,promote the occurrence of hypertension in pregnant women.Therefore,this study started from the placental microflora and used 16 S r RNA high-throughput sequencing technology to study the placental microflora of healthy(NP)pregnant women and preeclampsia(PE)pregnant women with non-specific inflammatory indicators: As a predictor of PE,NLR(neutrophil to lymphocyte count ratio)aims to provide new directions for the screening,treatment,and prognosis of PE.Research contents and methods:1.Specimen collection: A total of 60 eligible pregnant women were recruited from the First Affiliated Hospital of Nanchang University from October 2021 to October 2022.Patient information was recorded and peripheral blood,placenta and urine samples were collected.2.Sample processing: The placenta samples from two groups of pregnant women during cesarean section were collected under aseptic conditions,and bacterial DNA was extracted.The qualified genomic DNA samples were used as templates to amplify the V3-V4 variable region of 16 S r DNA and perform Illumina-Miseq high-throughput sequencing.The sequencing data were processed and ASV commentary analyzed.3.Data analysis: Alpha diversity analysis,Beta diversity analysis and LEf Se analysis were conducted on the data obtained at last.The peripheral blood routine indexes of PE group and NP group were detected.ROC curve and binary Logistic regression analysis were used to calculate the correlation between neutrophils/lymphocytes(NLR)and preeclampsia in blood routine.Research results:1.The ACE index and Shannon index of placental flora diversity in PE group were significantly lower than those in NP group(P < 0.05).β-diversity analysis showed that the placental flora of the two groups was significantly different(P <0.05).2.Phylum level: The relatively high abundance of placental flora in PE group and NP group included Bacteroidetes,Firmicutes,actinobacteria,Proteobacteria and flexicutes.Genus level: Muribaculaceae,Spirillum,Staphylococcus,Clostridium,Bacteroides,Pseudomonas,Mycobacterium,Staphylococcus,Clostridium,Escheric-hia coli Shigella,Rumen coccus,Klebsiella were relatively abundant in the two groups.3.LEf Se analysis showed that the flora abundance of Proteobacteria,Proteobacteria,Sphingomonas,Sphingosinomonas,Pseudomonas,Pseudomonas,Pseudomonas,Klebsiella,and Escherichia coli in PE group was higher.The relative abundance of Bacteroidetes,Muribaculaceae,Spirillaceae,Clostridium,Flavobacter-ium,Spirillum,Staphylococcus and Rumen cocci in NP group was higher.4.ROC curve and binary Logistic regression analysis showed that the NLR of PE group was significantly higher than that of NP group(P<0.05).When the NLR was 4.254,the sensitivity was 88.0% and the specificity was 74.3%.Research conclusion:This study found that there was a significant difference in placental flora between PE patients and NP women,and the richness and diversity of placental flora in PE patients decreased.At the genus level,the dominant bacteria of PE group were mainly concentrated in Proteobacteria,and the dominant bacteria of NP group were mainly concentrated in Firmicutes.Some potential pathogenic bacteria Fusobacterium,Escherichia-Shigella,Klebsiella and Alistipes,Muribaculaceae,Lachnospiraceae,Ruminococcus and others with high abundance in the placenta of PE patients The decrease of beneficial bacteria may be the key factor in the pathogenesis of PE patients,and the abnormal structure of these bacteria flora may lead to the occurrence of systemic inflammatory response.The NLR of PE group was significantly higher than that of NP group,and the good boundary point of NLR for the diagnosis of preeclampsia was 4.254.It can provide direction for prevention and treatment of PE etiology.