Application Of Imprint Cytology Combined With DNA Quantitative Analysis In Rapid Diagnosis Of Intraoperative Pulmonary Nodules

Objective:By changing the preparation conditions of DNA quantitative analysis,studying the application value of tissue imprint cytology combined with DNA quantitative analysis in the rapid pathological diagnosis of pulmonary nodules during operation,and exploring the correlation between cellular DNA content and clinicopathological parameters of non-small cell lung cancer.Methods:1.Firstly,the Feulgen staining reaction of human normal bronchial epithelial BEAS-2B cell line was observed by changing temperature,acidolysis time and staining time.2.Select the temperature with the best reaction speed above,explore the effect of acidolysis time on Feulgen staining reaction of human normal lung tissue imprint at this temperature,optimize other steps of Feulgen staining reaction,and summarize the improved Feulgen staining reaction process.3.The diagnostic test was used to compare the consistency between the results of routine Feulgen staining and modified Feulgen staining DNA quantitative analysis.4.To study the value of tissue imprint cytology,modified DNA quantitative analysis,and combined diagnosis in rapid pathological diagnosis during operation.5.To observe the value of imprint cytology combined with DNA quantitative analysis in the diagnosis of intraoperative frozen sections.6.The clinical data of patients were collected to explore the relationship between cellular DNA content and clinicopathological parameters of non-small cell lung cancer.Results:1.With the increase in temperature,the platform period of the dyeing reaction was shortened and the dyeing stability was weakened.2.When at least 4 positive cells appeared as the diagnostic cutoff point of modified Feulgen staining DNA quantitative analysis,the specificity was the same as that of conventional Feulgen staining DNA quantitative analysis,but the sensitivity was slightly lower,but the difference was not statistically significant.3.The sensitivity and specificity of imprint cytology combined with DNA quantitative analysis were 92.771% and 94.366% respectively.The sensitivity of combined diagnosis was higher than that of imprint cytology,and the specificity was higher than that of modified DNA quantitative score.The difference was statistically significant,which was highly consistent with the results of the frozen section diagnosis.4.The content of DNA in NSCLC was positively correlated with tumor size,histological grade,pleural invasion,lymph node,metastasis,and clinical stage,but not with age and airway spread.Conclusion:1.The modified DNA quantitative analysis has the same diagnostic value as the conventional DNA quantitative analysis,and the diagnostic results are more consistent.2.Imprint cytology combined with DNA quantitative analysis is of high diagnostic value,which has a certain reference value for rapid pathological diagnosis of intraoperative frozen sections.3.Cellular DNA content can be used as a potential detection index of the malignant degree of non-small cell lung cancer.