Effects Of Celecoxib On The Expression Of E-Cadherin,Vimentin,and β-Catenin In Rat Colorectal Cancer

Objective: To investigate the effect of celecoxib on the expression of EMT-related markers E-cadherin,Vimentin,and β-catenin in rat colorectal cancer tissues,which were used with different dose of celecoxib intervention.Methods: Thirty SD rats were randomly divided into 5 groups: control group,model group,low dose celecoxib intervention group,medium dose celecoxib intervention group,high dose celecoxib intervention group,with 6 rats in each group.The rats in the model group and each celecoxib intervention group were intraperitoneally injected with dimethylhydrazinel once a week,for a total of injections 20 weeks;Meanwhile,each celecoxib intervention group was given different concentrations of celecoxib solution intragastric perfusion,once a day,and all rats were killed after continuous administration for 28 weeks.The large intestine tissues were collected for HE staining to clarify whether the model of rat colorectal tumor was successfully established.According to the result of modeling,the large intestine tissues of rats in each group were selected for experiment.The expression levels of E-cadherin,Vimentin,and β-catenin proteins were detected by immunohistochemistry and Western blot,and the relative expressions of Vimentin andβ-catenin m RNA were detected by q RT-PCR.Results:1.Establishment of rat colorectal tumor model results: Colorectal tumors were formed in all experimental groups except in the six rats in the control group.2.Results of immunohistochemistry: The expression of E-cadherin protein was declined,while the expressions of Vimentin and β-catenin protein were augmented in the model group when compared to control group(P<0.05);Compared to the model group,the expression of E-cadherin protein was augmented,while the expressions of Vimentin andβ-catenin protein were declined in the high dose celecoxib intervention group(P<0.05);Compared with the low dose celecoxib intervention group,the expression of E-cadherin protein was augmented,while the expressions of Vimentin and β-catenin protein were declined in high dose celecoxib intervention group(P<0.05).3.Results of Western blot: The expression of E-cadherin protein was declined while the expressions of Vimentin and β-catenin protein were augmented in the model group compared with the control group(P<0.05);Compared with the model group,the expression of E-cadherin protein was augmented,while the expressions of Vimentin andβ-catenin protein were declined in medium dose and high dose celecoxib intervention group(P<0.05);Compared with the low dose celecoxib intervention group,the expression of E-cadherin protein was augmented,while the expressions of Vimentin and β-catenin were declined in high dose celecoxib intervention group(P<0.05).4.Results of q RT-PCR: The expressions of Vimentin and β-catenin m RNA were augmented in model group when compared to control group(P<0.05);Compared with the model group,the expressions of Vimentin and β-catenin m RNA were declined in the medium dose and high dose celecoxib intervention group(P<0.05);Compared with the low dose celecoxib intervention group,the expressions of Vimentin and β-catenin m RNA were declined in the medium dose and high dose celecoxib intervention group(P<0.05).Conclusion:1.The expression of E-cadherin was declined,while the expressions of Vimentin andβ-catenin were augmented in rat colorectal cancer tissues,indicating that EMT is involved in the process of colorectal carcinogensis in rats。2.Celecoxib can augment the expression of E-cadherin and decline the expressions of Vimentin and β-catenin in rat colorectal cancer in a dose-dependent manner,which suggests that celecoxib may inhibit EMT of colorectal cancer at the tissue level.