| Objective:To explore the correlation between the general clinical parameters(such as blood lipid,blood glucose,uric acid,etc.)of patients with colorectal polyps and colorectal cancer,compare the differences in gut microbiota between patients with different pathological types of polyps and colorectal cancer,and provide new ideas and strategies for early diagnosis and treatment of colorectal cancer.Methods:The patients who underwent colonoscopy in the provincial hospital affiliated with Shandong University from June 2021 to September were selected.(1)according to the examination results,the subjects were divided into case group and healthy control group(HC group),and the case group was divided into inflammatory polyposis group(IP group),hyperplastic polyposis group(HP group),adenomatous polyposis group(APC group)and colorectal cancer group(CRC group);(2)Collect the clinical data of each group,including age,sex,body mass index(BMI),previous medical history,personal history,etc.Collect serological samples and detect the following indicators:Uric Acid(UA),Triglyceride(TG),Total Cholesterol(TC),Triglyceride(TG),Low-Density Lipoprotein(LDL),High-Density Lipoprotein(HDL),Lipoprotein(a)(LPa),Total Bile Acids(TBA),Glycocholic Acid(GC),Fasting Blood Glucose(FBG),C-reactive protein(CRP),etc.(3)Collect the fecal samples of all the subjects,the V3-V4 region of bacterial 16S rDNA was amplified and sequenced;(4)Alpha and Beta diversity were calculated by QIIME software.(5)The effect size(LEfSe)of linear discriminant analysis(LDA)was utilized to find the bacterial taxa and metabolism-related clinical factors that differed significantly across groups;(6)Through nonparametric test and T-test,find out the different bacteria of each group at the level of family,genus and species;(7)Spearman correlation analysis to compare the correlation between intestinal flora and clinical parameters;(8)Construct a symbiotic network diagram for correlation analysis of microbiota.Results According to the standard of nano-excretion,71 subjects were analyzed,including 10 IP patients,9 HP patients,24 APC patients,19 CRC patients,and 9 HC people as controls.(1)Analysis of general clinical data showed that there were significant differences in GC,LPa levels,previous history of diabetes,and the size of colorectal polyps with different pathological types(P<0.05).The GC level in the CRC group was the highest,while the proportion of diabetic patients in the APC group was the highest.The polyp sizes in the IP group,HP group,and APC group showed an increasing trend.(2)In the CRC group,the abundance of Rikenellaceae,Erysipelotrichaceae and Alistipes were higher than in other groups,while in the IP group,butyric acid-producing bacteria were the dominant expression,and Phascolarctobacterium and Agathobacter were more abundant than that of other groups(P<0.05).(3)Spearman correlation analysis between the relative abundance of intestinal microflora and clinical parameters showed that ① Bacteroides and Streptococcus were positively correlated with total cholesterol level,while Enterococcus was negatively correlated with total cholesterol level.②The level of high-density cholesterol was positively correlated with Roseburia.③The level of glycocholic acid was positively correlated with Butyricimonas and negatively correlated with Faecalibacterium.④Fasting blood glucose levels was negatively correlated with Ruminococcus.Conclusion The level of glycocholic acid is the highest in colorectal cancer patients,and the proportion of diabetic history is the highest in adenomatous polyps.The maximum diameters of inflammatory polyps,hyperplastic polyps and adenomatous polyps are increasing.There was no significant difference in the richness of intestinal microflora among the groups,but the composition of dominant bacteria was different.The number of conditional pathogenic bacteria in intestinal microflora of patients with adenomatous polyp and colorectal cancer increased,while the number of beneficial bacteria in intestinal microflora of patients with inflammatory polyp increased.Intestinal flora has a significant correlation with biochemical indexes related to blood lipid and blood glucose metabolism,and intestinal flora may affect the incidence of colorectal cancer by participating in metabolism. |
