Study Of Epidemiology Survey And Genotyping Of Cutaneous Warts In A Unit Of PLA

Background and purpose:Cutaneous warts were common in dematological outpatient.A high proportion of the disease in military outpatients was show in literature,which may imply an higher prevalence of the disease in the PLA.This speculation needs to be confirmed by epidemiology survey.Epidemiology survey both home and abroad show that age,race,region and personal habits affect the prevalence of the disease.The genotype of the virus affects the clinical presentation,treatment choice,and prognosis of the disease.No consistent conclusions were draw about this tropic.we made an epidemiology survey to learn the prevalence of cutaneous HPV infection in a unit of PLA and to investigate the relevant risk factors of the disease.Some suggestions were given based on this study.We collected wart samples from servicemen in the outpatient department for HPV genotyping and explored the relationship between HPV types and their clinical manifestations.Section I: Epidemiology survey of Cutaneous warts in recruits of PLA.Methods:Taking the opportunity to participate in the physical examination of recruits in 2020-2021 and enter the camp to serve the military,we check the head,face,neck,hands and feet of recruits one by one to investigate the prevalence of Cutaneous warts.The electronic questionnaire as well as paper questionnaire was adopted to collect the general information and relevant messages of the participants.The characteristics of the patients with Cutaneous warts were recorded in detail.The possible risk factors of the disease were statistically analyzed by Chi-square test and multivariate logistic regression.Results:The prevalence rate of Cutaneous warts in recruits was 2.49%.Most of the patients were common and plantar warts,which were usually less than 1 cm in diameter and with mild discomfort.Multivariate logistic regression analysis showed that from single parent family,smoking,sharing personal items with others were risk factors,and the incidence risk increased by2.934、1.693 and 1.863 times respectively.The South China region was a protective factor,and the incidence was only 0.101 times.Section II: Epidemiology survey of Cutaneous warts in a unit of PLA.Methods:Taking the opportunity to participate in the annual physical examination of a certain troops,we check the head,face,neck,hands and feet of servicemen one by one to investigate the prevalence of Cutaneous warts.The electronic questionnaire was adopted to collect the general information and relevant messages of the participants.The characteristics of the patients with Cutaneous warts were recorded in detail.The possible risk factors of the disease were statistically analyzed by Chi-square test and multivariate logistic regression.Results:The prevalence rate of Cutaneous warts in servicemen of a certain troops was 4.37%.There was no significant difference in the prevalence rate between servicemen with different service years.The clinical characteristics of patients with Cutaneous warts are similar to those found in recruit surveys.Multivariate logistic regression analysis showed that direct contact with patients,history of having had cutaneous warts,smoking and sleep less than 6 hours a day or with poor sleep quality were risk factors,and the incidence risk increased by 7.820、7.491、1.640 and 1.968 times respectively.Section III: Determination of HPV type in specimens of cutaneous warts.Methods:Samples as well as general information and characteristics of the patients who suffered from cutaneous warts were all collected in the outpatient department.HPV L1 region were amplified and sequenced to determine HPV type based on the primer sequences reported in literature.The relationship between HPV types and their clinical manifestation were analyzedResults:182 specimens from 161 patients were collected,including 87 common warts,7 plane warts,65 plantar warts and 2 common warts as well as plantar warts.The most common HPV types were HPV27,1,2,4 and 57.HPV1 and HPV4 infection had significantly different clinical manifestation.Conclusions:1.The prevalence rate of cutaneous warts in recruits was 2.49%.Most of the patients were common and plantar warts,which were usually less than 1 cm in diameter and with mild discomfort.The risk factors for recruits were from single parent family,smoking and sharing personal items with others.The protective factor was from South China.2.The prevalence rate of cutaneous warts in serviceman of a certain troops was 4.37%,with no significant difference between servicemen of different service years.The risk factors for servemen were direct contact with the patients,history of having had cutaneous warts,smoking and sleep less than 6 hours a day or with poor sleep quality.3.The most common HPV types of Cutaneous warts in servicemen were HPV27,1,2,4and 57.HPV1 and HPV4 infection had significantly different clinical characteristics.