Analysis Of 2318 Cases Of Human Papillomavirus Genotyping And The Related Factors

Objective:To investigate the distribution of human papillomavirus genotypes,as well as its correlation with women's age and seasons,and to calculate the clearance time of the virus,in order to make a contribution to the theoretical basis of cervical disease prevention and treatment.Methods:A retrospective study of the results of HPV genotyping which were screened in the gynecological out-patients of Nanjing Drum Tower Hospital,out of cervical samples from 2318 volunteers were performed to work out the correlation between the distribution of the HPV genotypes and patients' age as well as seasons.Then We selected only the ones who were the first time to be found out HPV postive,and followed them up and redid HPV genotypes after 3 months until they became negative to calculate the HPV clearance time.Result:1.The results of HPV genotyping of 2318 cases:908 cases turned out negative,and 1410 positive(60.83%),the most common genotypes of high-risk HPVs were HPV16,58,52,31,53 and 33,while those of the low-risk HPVs were mainly HPV81(CP8304),11 and 6.2.The positive rates of high-risk HPV varied with age.1)? 53 years old group:537 cases,285 of which were high-risk HPV positive(53.07%).2)31-40 years old group:791 cases,431 of which were high-risk HPV positive(54.49%).3)41-50 years old group:642 cases,346 of which were high-risk HPV positive(53.89%).4)51-60 years old group:272 cases,121 of which were high-risk HPV positive(44.49%).5)? 61 years old group:76 cases,28 of which were high-risk HPV positive(36.84%).Statistical analysis of the positive rates among the groups found that,?30 years old group,31-40 years old group and 41-50 years old group,was statistically significant(P<0.05)to 51-60 years old group and?61 years old,respectively.There was no statistically significant difference(P>0.05)besides.3.The positive rates of high-risk HPV also varied with seasons.The total number and the high-risk HPV positive number in each season were 766 and 442(57.70%)in spring,628 and 356(56.69%)in summer,413 and 228(55.21%)in autumn,511 and 254(49.71%)in winter,respectively.Statistical analysis of the positive rates among the seasons found that There was a statistically significant difference between the positive rate in winter and that in spring or summer(P<0.05).There was no statistically significant difference(P>0.05)besides.4.The distribution of HPV genotypes in four seasons:1)The most common HPV types in spring were HPV16(12.92%),HPV58(12.14%),HPV52(9.92%)and HPV31(7.31%);2)The most common HPV types in summer were HPV16(16.88%),HPV58(9.55%),HPV52(9.39%)and HPV18(6.69%);3)The most common HPV types in autumn were HPV11(16.22%),HPV58(10.65%),HPV52(10.65%),HPV33(6.30%)and HPV18(4.73%);4)The most common HPV types in winter were HPV16(10.57%),HPV58(10.18%),HPV52(8.81%),HPV31(5.68%)and HPV53(5.68%).5.A total of 99 women who were the first time found HPV positive,and routinely redid HPV genotypes after 3 months till negative.There were 72 cases of high-risk HPV infection,and the average clearance time of HPV among those cases was 268.53 days.Particularly the average clearance time of HPV 16 among those cases was 272.23 days.There were 27 cases of first-found low-risk HPV infection,and the average clearance time was 219.19 days.The clearance time was contained within 3-12 months in 50%of all 99 high-risk HPV positive women while the median clearance time was 182 days.Conclusions:1.The most common high-risk HPV genotypes that screened in the gynecological out-patients of Nanjing Drum Tower Hospital were HPV16,58,52,31,53 and 33,while the most common low-risk HPV genotypes were HPV81(CP8304),11 and 6.2.The infection rate of high-risk HPV varied with age and the higher stage of age was<50 years old,while the rate declined after 51 years old.3.The positive rates of high-risk HPV also varied with seasons.The rate in spring or summer was higher than that in winter,and there was no statistically significant difference besides.4.The distribution of HPV types in four seasons are slightly different.HPV16/58/52 are most common in all seasons.In addition,HPV31 is common in spring,while HPV18 is common in summer,and HPV33 is common in autumn,5.In our study,the clearance time was contained within 3-12 months in 50%of all 99 high-risk HPV positive women while the median clearance time was 182 days.