Effects Of Different Temperature And Growth Regulators On Flower Bud Differentiation And Quality In Crocus Sativus L.

In this study,Crocus sativus L.was used as experimental material to study the effects of variable temperature treatment and gibberellin and 6-Benzylaminopurine on flower bud differentiation and quality of C.sativus.Paraffin section technology was used to observe the flower bud differentiation in each stage;growth indexes such as corm weight and bud length in indoor cultivation stage of C.sativus were measured and analyzed;the relationship between variable temperature treatment and growth regulator treatment and the contents of nutrients,antioxidant enzymes,endogenous hormones and active substances in corm was analyzed.The mechanism of flower bud differentiation regulated by variable temperature and growth regulators was revealed,and the combination of variable temperature and concentration of growth regulators were selected to promote flower bud differentiation and flowering,and improve stigma quality of C.sativus.It provides a theoretical basis for the high-yield cultivation and management techniques of C.sativus.The main conclusions are as follows:1.In the variable temperature treatment,through the observation of the apical bud sections of five different treatment groups,it was found that the corms treated with T1（20℃-25℃-20℃）had flower bud differentiation prior to other groups,and the first flowering time was one week earlier than other groups.2.In the variable temperature treatment,by analyzing the nutrient content,antioxidant enzyme activity and endogenous hormone content of corm in five different treatment groups,the results showed that compared with CK,the decomposition amount of starch and the accumulation of soluble protein in T1 treatment were more significant,while the starch content in T3（25℃-35℃-25℃）treatment decreased the least;The activities of SOD,CAT and POD in T1 treatment were significantly increased,while the activities of SOD,CAT and POD in T3 treatment were significantly lower than those in other groups;the contents of IAA and GA in T1 treatment were lower than those in other groups,while the contents of IAA and GA in T3 and CK treatment were higher than those in other groups.3.In the study of growth regulator,i.e.gibberellin and 6-Benzylaminopurine combined application,it was found that the starch content showed a downward trend,the soluble sugar and soluble protein content showed an upward trend,the antioxidant enzyme activity showed an upward trend,the endogenous hormone content showed an upward trend at first and then a downward trend,and the nutrient content of C.sativus in different treatment groups was higher than that in control group There were significant differences in antioxidant enzyme activity,endogenous hormone content and active substance content.The contents of crocin-Ⅰ,crocin-Ⅱand crocin in different treatment groups were also significantly different.4.Through the membership function analysis of C.sativus,the corm growth status of variable temperature treatment groups can be comprehensively evaluated,and the mean membership function of each treatment group is significantly higher than that of CK treatment group,which indicates that gibberellin and 6-Benzylaminopurine soaking treatment have great influence on the growth and active components of C.sativus,and the mean membership function of T13 is the largest,which indicates that this evaluation system is feasible The optimum concentration of GA and 6-BA was 150 mg·L^-1 GA and 50mg·L^-16-BA.5.In this experiment,in order to break the dormancy of C.sativus,promote early flower bud differentiation and improve the quality of C.sativus,the most suitable temperature was T1（20℃-25℃-20℃）,and the most suitable growth regulator was 150mg·L^-1 GA and 50 mg·L^-16-BA.